1200ITU/mg Bti浓悬浮剂的研制  

Development of 1 200 ITU/mg Bacillus thuringiensis subsp.israelensis Suspension Concentrate

作　　者：廖先清[1] 刘芳[1] 周荣华[1] 张遵霞[1] 杨自文[1] 

机构地区：[1]湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药研究分中心,武汉430064

出　　处：《湖北农业科学》2011年第24期5113-5115,共3页Hubei Agricultural Sciences

基　　金：国家科技支撑计划项目(2011BA40B00);湖北省农业科技创新中心资助项目(2007-620-001-03)

摘　　要：通过对苏云金芽孢杆菌以色列亚种Bti L-10菌株进行培养基的改良优化得到一种理化性质好、利于后处理的配方。200 L罐发酵效价达395 ITU/μL;通过后处理工艺的优化,得到一种制备1 200 ITU/mgBti浓悬浮剂路线,即发酵液2次离心并高倍浓缩得到菌浆,添加各种助剂经砂磨机研磨,得到Bti浓悬浮剂产品效价达到1 245 ITU/mg。The culture medium for highly toxic Bacillus thuringiensis subsp.israelensis L-10 strain was obtained by orthogonal tests of nitrogen source.The formula with good physicochemical properties was conducive to the post-treatment.In the pilot fermentation in 200 L fermentors,the potency of the fermentation broth was 395 ITU/μL.After the optimization of post-treatment,the optimal route of 1 200 ITU/mg Bti suspension concentrate was developed.It was prepared by using fermentation broth to the secondary centrifugation and high-powered concentration,then adding additive and grinding with sand mill.Its titer reached 1 245 ITU/mg against mosquito larva.

关 键 词：苏云金芽孢杆菌以色列亚种 浓悬浮剂 制备工艺 

分 类 号：S482.39[农业科学—农药学] TQ453.5[农业科学—植物保护]