农杆菌介导的氮高效利用转基因水稻植株的获得  

Transgenic High N Use Efficiency Rice Obtained by Agrobacterium tumefaciens

作　　者：查中萍[1] 万丙良[1] 殷得所[1] 杜雪树[1] 戚华雄[1] 

机构地区：[1]湖北省农业科学院粮食作物研究所,武汉430064

出　　处：《湖北农业科学》2011年第24期5247-5249,共3页Hubei Agricultural Sciences

基　　金：湖北省科技厅国际合作项目(2010BFA019)

摘　　要：将从深海硅藻中克隆的与氮具有高度亲和性的硝酸盐转运蛋白基因NAT3通过农杆菌介导的遗传转化方法,转入水稻品种中花11,在含30 mg/L Basta的选择培养基上筛选,获得456棵抗性植株,对NAT3目标基因检测,获得313棵阳性植株,初步证明目标基因已整合到中花11基因组中。NAT3 gene coding protein with high nitrate combining ability cloned from Cylindrotheca fusiformis was transmitted into rice ＇zhonghua 11＇ via Agrobacterium-mediated transformation.456 regenerated rice plants with resistance were obtained by screening on the culture medium with 30 mg/L basta.313 positive transgenetic plants were gained by PCR amplification analysis targeting at NAT3 gene.It was confirmed that NAT3 gene had been integrated into the genome of ＇zhonghua 11＇.

关 键 词：转基因水稻 硝酸盐转运蛋白 氮高效利用 

分 类 号：S511[农业科学—作物学] Q78[生物学—分子生物学]