细粒棘球绦虫高免动物血清的特异性和敏感性分析  

Echinococcus Granulosus High from Animal Serum Specificity and Sensitivity

作　　者：闫昊[1,2] 张旭[3] 古努尔.吐尔逊 米晓云[3] 石保新[3] 薛晶[3] 巫剑[3] 赵莉[3] 李丹[1] 张睿 张壮志[3] 

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [2]新疆维吾尔自治区动物卫生监督所,新疆乌鲁木齐830063 [3]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000

出　　处：《草食家畜》2011年第4期76-79,共4页Grass-Feeding Livestock

基　　金：公益性行业科研专项(日本血吸虫病;包虫病;弓形虫病;附红细胞体病等主要人畜共患寄生虫病的防控技术研究)项目编号:200903036-12

摘　　要：目的获得用于诊断细粒棘球绦虫成虫感染时特异性和敏感性高的抗血清。方法用细粒棘球绦虫成虫抗原免疫兔2只,制备高免血清,血清用饱和硫酸铵沉淀、透析、获得比较纯化的抗体,测量浓度和效价后,3μg/ml兔抗体包被,检测感染了不同犬带科绦虫的实验犬和空白犬粪样,单克隆抗体补捉抗原(1∶6000),再用HRP标记的兔抗小鼠IgM(1∶2000),OD 405nm读值,用以判定兔高免血清的特异性和敏感性。结果高免血清抗体效价均为16万,其敏感性为85%(34/40),特异性为87.5%(42/48)。结论此高免血清可以用于建立高敏感性和高特异性的诊断细粒棘球绦虫成虫感染的ELISA方法的建立和优化。Objective Get used to diagnose echinococcus grantxlosus adlxlt infection in the specificity and sensitivity high serum protein resistance. Methods Eehinoeoccus grantxlosus adult antigen immune rabbit 2 only,Preparation high serum free, Serum with 45% of the saturated ammonium slxlfate precipitation, dialysis, obtain purified antibodies, After measuring concentration and titer, 3μg/ml rabbit antibody bag was , Detection infected with families of different dogs experimental dogs and blank tapeworms dog dung samples , Monoelonal antibody capture antigen （1：6 000） , Garnish with the rabbit HRP markers in mice resistance IgM （1：2000）, OD 405nm read values, To determine the specificity of high rabbit serum free and sensitivity. Results The results High serum free antibody titer all be 16 million, The sensitivity is 85% （34/40）, specificity of 87.6% （42/48）.Conclusion This hyperimmune serum can be used to create high sensitivity and high specificity of the diagnosis of adult Echinococcus infection ELISA method development and optimization.

关 键 词：细粒棘球绦虫 高免血清 粪抗原 夹心法-ELISA 

分 类 号：S855.99[农业科学—临床兽医学]