罗汉果种子传播小西葫芦黄化花叶病毒的检测  

Detection of Zucchini yellow mosaic virus Transmission through Seeds of Siraitia grosvenorii

作　　者：朱英芝[1,2] 陈保善[2,3] 蒙姣荣[2,3] 

机构地区：[1]广西大学农学院,南宁530004 [2]亚热带农业生物资源保护与利用国家重点实验室,南宁530004 [3]广西大学生命科学与技术学院,南宁530004

出　　处：《基因组学与应用生物学》2011年第6期678-681,共4页Genomics and Applied Biology

基　　金：广西自然科学基金项目(桂科自0832045);广西创新能力建设项目(桂科能05112001-1B;桂科能05112001-1)共同资助

摘　　要：为了解小西葫芦黄化花叶病毒(Zucchini yellow mosaicvirus,ZYMV)是否能通过罗汉果种子传播。本研究通过取ZYMV阳性的罹病罗汉果植株种子700粒,400粒直接播种于防虫网室中,300粒经表面消毒处理后播种于MS培养基上。最终获得213棵罗汉果实生苗。对所获得实生苗进行生物学鉴定及ZYMV特异性RT-PCR检测,均未检测到ZYMV阳性植株。结果表明ZYMV不能经罗汉果种子传播,或其传播率很低。In order to understand whether the Zucchini yellow mosaic virus （ZYMV） transmitted through seeds of Siraitia grosvenorii. A total of 700 seeds were collected from ZYMV-infected plants. Four hundred of 700 seeds were cultivated directly in net-house for control pest, and 300 were cultivated in MS medium after surface steril- ization with alcohol. ZYMV of young plantlets were detected by observation or RT-PCR with ZYMV-specific primers. Two hundred and thirteen young plantlets were survived from 700 seeds. No ZYMV-positive sample was detected by observed or RT-PCR with ZYMV-specific primers. The results indicated that ZYMV could not be transmitted via seeds ofSiraitia grosvenorii, or the transmission rate was very low

关 键 词：罗汉果 小西葫芦黄化花叶病毒 RT-PCR 种子 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]