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机构地区:[1]东北油田盐碱植被恢复与重建教育部重点实验室,东北林业大学盐碱地生物资源环境研究中心,哈尔滨150040 [2]东京大学亚洲生物资源环境研究中心,东京1880002
出 处:《基因组学与应用生物学》2011年第6期703-706,共4页Genomics and Applied Biology
基 金:国家林业局948项目(2008-4-29);黑龙江省杰出青年科学基金(JC200609)共同资助
摘 要:为了比较分析盐碱土壤与非盐碱土壤微生物资源抗盐碱性差异,本研究利用含有不同浓度Na2CO3、NaHCO3和pH的培养基对盐碱土壤和非盐碱土壤细菌进行培养计数。结果显示:非盐碱土壤出菌数量随Na2CO3、pH和NaHCO3浓度升高而下降,盐碱土壤细菌出菌数量随着Na2CO3、pH和NaHCO3浓度升高先是升高然后下降,最高值分别出现在200mmol/LNaHCO3、50mmol/LNa2CO3和pH9.0的分离平板上。此外,高Na2CO3、pH和NaHCO3浓度的平板中盐碱土壤出菌数量远高于非盐碱土壤;以上结果可见,耐盐碱细菌资源主要集中分布在盐碱土壤中,在非盐碱土壤中虽有分布,但是仅占有很少一部分。To compare the different tolerance to the salt and alkali stresses of microbial resources in saline-alkali soil and non-saline-alkali soil, the bacteria among the soils were cultured in the medium containing different concentrations of Na2CO3 and NaHCO3 and different values of pH. The results showed that the bacteria count decreased in the non-saline-alkali soil, but increased at first and then decreased in the saline-alkali soil, with the increase of the concentrations of NaECO3 and NaHCO3 and the values of pH. As the result showed, the bacteria count reached to the summit in the saline-alkali soil at the following conditions, NaHCO3 200 mmol/L, NaECO3 50 mmol/L and pH 9.0. Additionally, at the same stress condition, the bacteria species were much more in the saline-alkali soil than that of non-saline-alkali soil. The results above indicated that the tolerant bacteria to saline and alkali stress distributed mainly in the saline alkali soil and only a few of tolerant bacteria species existed in the non-saline-alkali soil.
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