银杏叶提取物对枯否细胞表达肿瘤坏死因子-α的影响  

Ginkgo biloba extract inhibits tumor necrosis foctor-α expression in LPS-induced rat Kupffer cells

作　　者：王国栋[1] 袁同洲[1] 许诺[1] 黄野[1] 

机构地区：[1]南京医科大学第二附属医院骨科,江苏南京210011

出　　处：《吉林医学》2012年第1期10-12,共3页Jilin Medical Journal

摘　　要：目的:观察银杏叶提取物对枯否细胞(KC)分泌和表达肿瘤坏死因子-α(TNF-α)的影响。方法:用脂多糖刺激大鼠肝脏来源的KC,与不同浓度的银杏叶提取物进行培养,采用逆转录多聚酶链式反应(RT-PCR)和酶联免疫吸附测定(ELISA)方法分别检测枯否细胞TNF-αmRNA和蛋白质表达。结果:银杏叶提取物对大鼠肝脏来源的KC的TNF-α及其蛋白质表达呈浓度依赖性,随着银杏叶提取物的浓度的增加,TNF-α的mRNA及其蛋白质表达逐渐减少。结论:银杏叶提取物可以通过调节枯否细胞表达TNF-α发挥抗炎保护肝细胞的作用。Objective To investigate the effect of Ginkgo biloba extract（GBE） on the expression of tumor necrosis foctor-α（TNF-α） in rat Kupffer cells（KC） induced by lipopolysaccharide（LPS）.Method The protein and mRNA levels of lipopolysaccharide（LPS）-induced TNF-α was determined in Kupffer cells using ELISA and RT-PCR respectively.Results GBE inhibited the expression of TNF-α concentration dependently.Conclusion The mechanism of GBE of blocking liver cell injuries induced by LPS may be correlated with inhibiting the excessive TNF-α by Kupffer cells.

关 键 词：枯否细胞 银杏叶提取物 脂多糖 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]