新疆杏品种SFB基因的克隆及实时定量表达  

Cloning and real-time fluorescence quantitative expression of SFB genes from apricot cultivars in Xinjiang area

作　　者：王大江[1] 冯建荣[1] 刘月霞[1] 姜新[1] 曹晓艳[1] 赵宝龙[1] 

机构地区：[1]新疆石河子大学农学院,新疆石河子832003

出　　处：《果树学报》2012年第1期60-65,共6页Journal of Fruit Science

基　　金：国家自然科学基金(30760143);科技部"863"计划(2008AA10Z109);教育部"新世纪优秀人才"支持计划(NCET-08-0918)

摘　　要：以新疆杏27个品种花粉为材料,进行转录水平SFB基因的克隆与测序;选取冬杏、苏陆克、库尔勒托拥3个杏品种,对其进行花粉离体培养,分析SFB基因在不同时期的表达特性。结果表明,在27个品种中扩增出4种大小不同的条带,引物组合1在26个品种中扩增出条带,其中品种贝新纳尔,赛买提的片段大小为447 bp;大优佳的片段大小为446 bp,其余品种的大小为434 bp。引物组合2在品种托乎提中扩增出大小为660 bp大小的条带。序列比对显示27个品种的SFB基因为20种基因序列,与其他物种SFB基因序列比对显示20种基因序列均为新SFB基因序列,在GenBank上的登录号为:HQ148064-HQ148083;SFB基因在3个杏品种花粉离体培养不同培养时期均有表达,其表达丰度的变化趋势一致,呈先增后减的趋势,均在培养24 h时表达丰度最高,但是每个品种的表达峰值不同。The pollens from 27 apricot cultivars in Xinjiang area have been used to clone and sequence the SFB gene in transcription levels. The pollens of Dongxing, Suluke, Kaertuoyong were cultured in Vitro and the expression characteristics of SFB gene in different periods were analyzed. The results showed that four different size fragments were cloned from 27 apricot cuhivars. The fragments were cloned from 26 cuhivars by primer combination 1.The fragments of Beixinnaer and Saimaiti were 447 bp. The fragments of Dayou- jia were 446 bp, and the others were 434 bp. The fragment which was 660 bp was cloned from Tuohuti by primer combination 2. Sequence alignment showed the SFB genes of 27 cultivars were 20 sequences. The sequence alignment with other cultivars showed that the 20 sequences were new SFB sequence, the acces- sion number of GenBank were HQ148064-HQ148083. SFB genes in pollen of 3 apricot cultivars were ex- pressed during the 4 different culture period, the transcript abundance showed increase at first and then decrease, the transcript abundance at the stage of 24 h in vitro culture was highest in all cuhivars, howev- er, the transcript peak abundance of 3 cultivars were different.

关 键 词：杏 自交不亲和 SFB基因 实时定量PCR 

分 类 号：S662.2[农业科学—果树学]