化瘀克塞胶囊预处理对局灶性脑缺血大鼠神经功能的影响及机制探讨  

Effect on nerve function of Hua Yu Ke Sai capsule pretreatment in cerebral ischemia rats

作　　者：程广清[1] 李凤玲[1] 李长生[1] 杨晓妮[1] 王逢猛[2] 

机构地区：[1]山东省千佛山医院,济南250014 [2]山东中医药大学

出　　处：《山东医药》2011年第50期12-13,共2页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(Y2005C43)

摘　　要：目的观察化瘀克塞胶囊预处理对局灶性脑缺血大鼠神经功能的影响,并探讨其可能的机制。方法将40只Wistar大鼠随机分为A、B、C、D组,造模前7 d开始灌胃。C、D组给予化瘀克塞胶囊12.2、24.4 g/kg,2次/d;A、B组则给予等体积的生理盐水。B、C、D组采用线栓法制备大脑中动脉缺血模型,A组只显露大脑中动脉但不栓塞。持续性缺血6 h后,行大鼠行神经功能缺损评分,检测血浆中的内皮素(ET)、一氧化氮(NO)。结果 A、B、C、D组神经功能缺损评分分别为0、(3.07±0.73)、(1.96±0.64)、(1.22±0.67)分,血浆NO水平分别为(58.03±5.09)、(36.98±9.66)、(47.52±7.88)、(51.87±7.34)μmol/L,血浆ET水平分别为(215.75±23.34)、(326.28±35.5)、(244.85±17.55)、(227.15±23.69)pg/ml;各组间两两比较,P均<0.05。结论化瘀克塞胶囊可通过提高局灶性脑缺血大鼠血浆NO水平、减少血浆ET的释放,促进神经功能能恢复。Objective To investigate the protective effects and its possible mechanisms of Hua Yu Ke Sai capsule pretreatment on the brain after ischemia injury in rats. Methods 40 rats were randomely divided into A, B, C and D group. Rats in C, D groups were treated by Hua Yu Ke Sai capsule（ 12.2, 24.4 g/kg） 7 days before MCAO, 2/day; whereas A, B groups were given NS. 6 hours after ischemia, rats in each group were used to estimate the neurological deficit score and determination of ET and NO. Results The neurological deficit score of A, B, C and D group was 0,3.07 ±0. 73, 1.96± 0.64, 1.22 ±0.67 ; the expression of NO was （58.03 ±5.09）, （ 36.98 ±9.66）, （47.52 ±7.88 ）, （ 51.87 ±7.34） μmol/L; the expression of ET was （215.75 ±23.34）, （326.28 ± 35.5 ）, （244.85 ±17.55 ）, （227.15 ±23.69） pg/ml. The differences among A, B, C, D group were significantly remarkable （ all P 〈 0.05 ）. Conclusion Hua Yu Ke Sai capsule can protect the brain from isehemia injury, and the neuro protections mechenism may be related to the decrease of ET and the increase of NO.

关 键 词：化瘀克塞胶囊 一氧化氮 内皮素 局灶性脑缺血 大鼠 

分 类 号：R651.1[医药卫生—外科学]