急性脑梗死患者IL-18基因启动子607C/A、137G/C位点多态性观察  

Observation of IL-18 gene promoter 607C/A and 137G/C locus polymorphisms in acute cerebral infarction patients

作　　者：李秀芹[1] 吴孟海[1] 秦爱丽[1] 高勇[1] 

机构地区：[1]莱芜市人民医院,山东莱芜271100

出　　处：《山东医药》2011年第50期14-15,共2页Shandong Medical Journal

摘　　要：目的观察急性脑梗死患者白细胞介素18(IL-18)基因启动子607C/A和137G/C位点单核苷酸多态性(SNP)。方法采用型特异性引物聚合酶链反应(PCR-SSP)技术检测98例脑梗死患者(观察组)和100例健康对照者(对照组)血清IL-18基因启动子607C/A和137G/C位点多态性。结果两组607C/A位点基因型CC、CA和AA的频率及等位基因频率相比P均>0.05。观察组组137G/C位点GG型频率显著高于对照组,GC型的频率显著低于对照组(P<0.05),两组等位基因频率的分布也有显著性差异(P<0.05)。结论急性脑梗死患者IL-18基因启动子607C/A位点SNP与脑梗死无关,137G/C位点携带等位基因C可能有预防急性脑梗死的作用。Objective To observe the single nucleotide polymorphisms （SNPs） located at 607 and 137 in the promoter region of serum interleukin-18 （IL-18） gene in acute cerebral infarction （ACI）patients. Methods Polymerase chain reaction with sequence specific primer （PCR-SSP） was used to genotype the SNPs of 607C/A and 137G/C in 98 ACI patients （observe group）and 100 healthy controls（ control group）. Results There were no significant difference in the distribution of genotypic frequencies and allelic frequency at position 607. The frequencies of 137 GG genotype was significantly higher in ACI patients （ P 〉 0. 05 ）, while 137 GC was significantly decreased in ACI patients compared to the control groups （ P 〈 0.05）. Significant difference was also found in aiielic frequency at position 137 between ACI patients and control （P 〈 0.05）. Conclusion The IL-18 promoter gene polymorphism at position 607 is not associated with ACI, but the polymorphism at position 137 is associated with ACI and C allele may be protected against ACI.

关 键 词：细胞因子 白细胞介素18 脑梗死 单核苷酸基因多态性 

分 类 号：R651.1[医药卫生—外科学]