大鼠30%小体积肝移植模型两种切肝方法的比较  

Comparison between two methods of liver lobe resection for a rat model of 30% small-for-size liver transplantation

作　　者：宋军[1] 丁志龙[1] 徐为[1] 李文美[1] 李向农[1] 王学浩[2] 

机构地区：[1]徐州医学院附属医院,江苏徐州221002 [2]南京医科大学第一附属医院

出　　处：《山东医药》2011年第52期16-18,共3页Shandong Medical Journal

基　　金：江苏省卫生厅科技项目(KF200917);徐州医学院院长基金(2010KJZ22;09KJZ10)

摘　　要：目的探讨建立理想的成功率较高的大鼠30%小体积肝移植模型的可靠方法。方法采用改良"二袖套法"非动脉化法建立大鼠30%小体积肝移植模型。实验组分为两组:A组,右叶+三角叶+尾状叶做供肝的30%小体积肝移植组,共40例;B组,中叶做供肝的30%小体积肝移植组,共40例。观察两组术后并发症和术后3 d生存率。结果 A、B组切肝时间分别为(11±3)、(14±3)min,P<0.01;术后发生肝后下腔静脉狭窄分别有2例、8例,P<0.05;术后3 d生存率分别为53%(21/40)、40%(16/40),P>0.05;断面出血、胆漏和感染的发生率两组无明显差异。结论通过技术改良,可以以右叶+三角叶+尾状叶为供肝建立稳定的大鼠30%小体积肝移植模型。Objective To establish an effective and available 30% small-for-size rat liver transplantation model.Methods 30% small-for-size liver transplantation models were performed according to Kamada＇s two-cuff method without arterial reconstruction.Male SD rats were divided into two groups： group A（n=40）,30% liver transplantation using right lobe,caudate lobe and triangle lobe graft;group B（n=40）,30% liver transplantation using a median lobe graft.Three-day survival and technical complications were compared between two groups.Results Compared to group B,the time for liver lobe reduction in the group A was shorter（P0.01）,and inferior vena cava stricture was fewer（P0.05）.There was no significant difference in the three-day survival rate between two groups（P0.05）.The incidence rate of liver cross-section bleeding,bile leakage and infection showed no significant differences.Conclusion Using improved method of lobe resection,a more viable and effective 30% small-for-size rat liver transplantation model with a right lobe,caudate lobe and triangle lobe graft can be established.

关 键 词：肝移植 模型 动物 小体积 大鼠 

分 类 号：R657.3[医药卫生—外科学]