高效液相色谱法测定胆石通胶囊中黄芩苷的含量  被引量:5

Determination of Baicalin in DanShiTong Capsules by HPLC

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作  者:夏小叶 

机构地区:[1]湖南省常德市药品检验所,湖南常德415000

出  处:《中国医药指南》2012年第1期39-41,共3页Guide of China Medicine

摘  要:目的建立胆石通胶囊中黄芩苷的测定方法,以有效地控制制剂的质量。方法采用高效液相色谱法(HPLC)测定胆石通胶囊中黄芩苷的含量。色谱柱为Promosil C18柱(4.6×250mm,5μm);以甲醇-0.3%磷酸(20:80)为流动相,流速为1.0mL/min,检测波长为276nm,柱温为25℃,采用外标法定量。结果黄芩苷线性范围为0.054-1.08ug,r=0.9997(n=5),平均回收率为98.2%,RSD为1.1%(n=9)。结论该方法简便、可靠、快速、重现性好,可用于胆石通胶囊中黄芩苷的含量测定。[Abstraetl Objective To establish a method for the content determination of baicalin in DanShiTong Capsules, so as to effectively control its quality. Methods The content of baicalin was detected by HPLC. The operating conditions were Promosil C18 (4.6×250 ram, 5μm) column, The mobile phase consisted of methanol-0.3% phosphoric acid (20 : 80). The detection wavelength was at 276 nm, the flow rate was 1.0mL/min, the column temperature was 25℃, external standard method was employed. Results The linear ranges was obtained between 0.054-1.08μg(r=0.9997,n=5) of baicalin. The average recovery rate was 98.2% respectively with corresponding RSD of 1.1%(n=9). Conclusion The method is simple, convenient,rapid and reliable with good reproducibilty, Which can be used for the content determination of baicalin in DanShiTong Capsules

关 键 词:胆石通胶囊 黄芩苷 高效液相色谱法 含量测定 

分 类 号:R282.710.5[医药卫生—中药学]

 

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