精氨酸和谷氨酰胺对猪小肠上皮细胞增殖的影响及机理探讨  

Effect of Arginine and Glutamine Addition on the Proliferation of Porcine Intestinal Epithelial Cell and Its Mechanism

作　　者：聂新志[1] 蒋宗勇[1] 林映才[1] 郑春田[1] 周桂莲[1] 汪仕奎[1] 王丽[1] 

机构地区：[1]广东省农业科学院畜牧研究所/畜禽育种国家重点实验室/农业部华南动物营养与饲料重点实验室/广东省动物育种与营养公共实验室/广东省畜禽育种与营养重点实验室,广州510640

出　　处：《中国农学通报》2012年第2期1-5,共5页Chinese Agricultural Science Bulletin

基　　金：国家现代农业产业技术体系建设专项资金"生猪"(177);国家联合基金"精氨酸调控新生仔猪小肠粘膜发育的机理研究"(u0731002);国际科技合作项目"母仔猪生产潜力营养调控关键技术研究"(2009DFA31570)

摘　　要：为研究精氨酸和谷氨酰胺对猪小肠上皮细胞增殖的影响,并探讨精氨酸和谷氨酰胺在小肠上皮细胞内的相互转化关系,在猪小肠上皮细胞培养液中添加不同剂量的精氨酸和谷氨酰胺,测定了细胞增殖、细胞中精氨酸合成代谢途径关键酶(P5CS、NAGS、NOS)基因mRNA表达量以及细胞上清液中精氨酸代谢产物(谷氨酰胺、精氨酸、精胺、一氧化氮)含量。结果表明:添加谷氨酰胺提高了细胞中NAGS基因mRNA表达量,有效提高了细胞中精氨酸含量,促进了细胞增殖;添加精氨酸降低了细胞中P5CS和NAGS基因mRNA表达量,抑制了猪肠上皮细胞中谷氨酰胺向精氨酸转化,并且,高剂量精氨酸显著降低了细胞上清液中谷氨酰胺含量;精氨酸与谷氨酰胺同时添加,对猪小肠上皮细胞增殖的促进效果优于两者单独添加。表明在猪小肠上皮细胞中,外源添加的精氨酸不能转化为谷氨酰胺,外源添加的谷氨酰胺可以转化为精氨酸并部分替代精氨酸发挥作用。To study the effect of arginine and glutamine on the proliferation of porcine intestinal epithelial cell and investigate the transformation between arginine and glutamine, the test added different doses of arginine and glutamine in the cultures of porcine intestinal epithelial cell. Measured cell proliferation, and mRNA expression of PSCS, NAGS, NOS gene in cells, and the content of glutamine, arginine, spermine, nitric oxide in the supernatants. The results were as follows： glutamine supplementation increased the mRNA expression of NAGS gene, increased the content of arginine in ceils, promoted the proliferation of cells. Arginine supplementation reduced the mRNA expression of P5CS and NAGS gene, inhibited the transformation from arginine to glutamine, and high-dose arginine supplementation significantly reduced the content of glutamine in the supernatant of ceils. The combined supplementation of arginine and glutamine than alone had better effect on proliferation of porcine intestinal epithelial cell. These results suggested that glutamine transferred to arginine and partial instead of arginine to play a role in porcine intestinal epithelial cell but arginine did not transfer to glutamine.

关 键 词：猪 小肠上皮细胞 精氨酸 谷氨酰胺 

分 类 号：S828[农业科学—畜牧学]