中国鹿亚科动物mtDNA控制区序列差异及其遗传分化  

Sequence Difference of Mitochondrial DNA Control Region and Genetic Differentiation of Cervinae in China

作　　者：涂剑锋[1] 邢秀梅[1] 徐佳萍[1] 杨颖[1] 杨福合[1] 

机构地区：[1]中国农业科学院特产研究所吉林省特种经济动物分子生物学重点实验室,吉林吉林132109

出　　处：《安徽农业科学》2012年第2期669-672,共4页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑计划(2007BAI38B03);国家自然科技资源平台项目(No.2004DKA30460)

摘　　要：[目的]探讨中国鹿亚科动物的遗传分化。[方法]通过PCR直接测序的方法获得我国鹿亚科物种线粒体DNA控制区(D-loop)全序列,结合GenBank检索到的鹿亚科其他动物同源序列,用生物软件进行统计分析。[结果]我国鹿亚科动物D-loop区序列全长在921～1 072 bp,碱基T、A、C、G的平均含量分别为32.1%、30.2%、22.7%及15.0%,各物种间的遗传距离范围在0.062～0.106之间,处于属间差异。系统进化树结果表明梅花鹿、马鹿和白唇鹿亲缘关系较近,它们与水鹿构成一组进化枝,坡鹿与麋鹿构成一组进化枝,豚鹿单独构成一个进化枝。[结论]该研究支持麋鹿和豚鹿并入鹿属的观点,我国的鹿亚科动物的分化时间约为155～260万年。[Objective] The study aimed at analyzing Genetic Differentiation of Cervinae.[Method] The complete mitochondrial DNA control region of 5 species of Cervinae was determined by direct DNA sequencing,Combined with homologous sequences of others Cervinae downloaded from GenBank,then analyzed through biology sofeware.[Conclusion] The lengths of their control region were 921-1 072 bp,the nucleotide content of T,A,C,and G was 32.1%、30.2%、22.7% and 15.0% separately,genetic distance of each species ranged from 0.062-0.106,they belonged to level of inter-genus.the molecular phylogenetic tree indicated that red deer,skia deer and White-lipped Deer had close relationship,they constituted one branch with sambar deer,Eld’s d eer and elk deer constituted the second branch,and hog deer constituted the third branch alone.[Result] Hog deer and fallow deer should be incorporated in cervus,divergence time were about 1.55-2.60 million years among Cervinae in China.

关 键 词：鹿亚科 控制区 序列差异 遗传分化 

分 类 号：Q96[生物学—昆虫学]