铁皮石斛种子液体悬浮培养的研究  

Liquid Suspension Culture of the Seeds of CAULIS DENDROBII

作　　者：史俊[1] 赵荣[1] 

机构地区：[1]江苏农林职业技术学院,江苏句容212400

出　　处：《安徽农业科学》2012年第2期727-728,737,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究铁皮石斛种子的液体悬浮培养方法。[方法]以MS为基础培养基,设计了不同浓度的基础培养基、激素、天然附加物、pH、蔗糖等5个影响因子的15种培养液配方,筛选出铁皮石斛最适的液体培养条件。[结果]适中的无机盐浓度,NAA、较低的pH值、较高的蔗糖浓度以及适量的土豆汁都有利于铁皮石斛种子的萌发;试验优选出的相对适合种子萌发的培养基为1/2MS+0.5 mg/LNAA+80 g/L土豆汁+45 g/L蔗糖。[结论]筛选出了相对较合适的培养基配方,为铁皮石斛栽培种植提供了理论依据。[Objective] To study on the liquid suspension culture for the seeds of CAULIS DENDROBII.[Method] Taking MS as the basic medium,15 formula of culture liquids with 5 influencing factors were designed,which were basic medium,hormone,natural additives,pH value,and sucrose.The optimal liquid culture condition for CAULIS DENDROBII was screened.[Conclusion] Moderate inorganic salt concentration,NAA,low pH value,relatively high sucrose concentration,and moderate potato juice were helpful to the seed germination of CAULIS DENDROBII.The culture medium suitable for the seed germination was 1/2MS＋0.5 mg/L NAA＋80 g/L potato juice ＋45 g/L sucrose.[Conclusion] Suitable culture medium was selected,which provided theoretical foundation for the cultivation of CAULIS DENDROBII.

关 键 词：铁皮石斛(cAuus DENDROBII) 种子 液体培养 

分 类 号：R282.27[医药卫生—中药学]