苦豆子渣中总黄酮含量的测定  

Determination of the Content of Total Flavonoids in Sophora alopecuroides L. Residues

作　　者：叶学军[1] 李力[2] 

机构地区：[1]宁夏专利服务中心,宁夏银川750001 [2]北方民族大学化学与化学工程学院,宁夏银川750021

出　　处：《安徽农业科学》2012年第2期736-737,共2页Journal of Anhui Agricultural Sciences

基　　金：宁夏回族自治区自然基金(NZ0845);国家民委重点开放试验室基金(2008SY15)

摘　　要：[目的]建立苦豆子渣中总黄酮含量的测定方法,为苦豆子再利用提供依据。[方法]采用NaNO2-Al(NO3)3-NaOH分光光度法测定苦豆子渣中黄酮的含量。[结果]回归方程为Y=11.403X+0.0021,R=0.999 8,表明芦丁浓度与吸光度呈良好的线性关系;加样回收率为98.67%～100.33%,RSD为1.65%(n=9);测定苦豆子渣中黄酮的平均含量为3.90 mg/g。[结论]建立的方法简便快速、成本低,可用于苦豆子渣中总黄酮的含量测定。[Objective] To establish a method for determining the content of total flavonoids in residues,so as to provide basis for the reuse of Sophora alopecuroides L.[Method] The content of total flavonoids in S.alopecuroides residues was determined by NaNO2-Al（NO3）3-NaOH spectrophotometry.[Result] The regression equation was Y=11.403X＋0.002 1,R=0.999 8,confirming a good linear relationship between the concentration and Absorbance of rutin.The recovery of total flavonoids ranged from 98.67% to 100.33% with the RSD of 1.65%（n=9）.In addition,the average content of total flavonoids in S.alopecuroides was determined as 3.90 mg/g.[Conclusion] The method is easy,convenient and low cost,which can be used for determining the content of total flavonoids in S.alopecuroides residues.

关 键 词：苦豆子渣 总黄酮 分光光度法 

分 类 号：R284.1[医药卫生—中药学]