HPLC法测定留兰香油中柠檬烯  

Determination of Limonene in Spearmint Oil by HPLC Method

作　　者：张瑞伦[1] 张庆[1] 徐世艾[1] 

机构地区：[1]山东省烟台大学化学工程与过程重点试验室化工制造工程高校重点试验室,山东烟台264005

出　　处：《安徽农业科学》2012年第2期743-744,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金资助项目(20476086;10272093);国家"863"重点资助项目(2006A030203);山东省自然科学基金重点资助项目(Z2007B04)

摘　　要：[目的]建立高效液相色谱法测定留兰香油中柠檬烯的含量。[方法]以无水甲醇为外标物,对留兰香油中柠檬烯的含量进行测定。色谱条件:色谱柱为C18(4.6 mm×250 mm,5μm),流动相为无水甲醇-水(75∶25,V/V),流速为1.0 ml/min,检测波长为200 nm,柱温为25℃。[结果]柠檬烯含量在0.21～1.05 mg/ml时,其峰面积与浓度呈线性关系,相关系数R2为0.995,平均回收率为98.0%,RSD为2.6%;计算得留兰香油原料中柠檬烯的含量为24.2%。[结论]该方法简单快速、准确,可用于留兰香油中柠檬烯的含量测定。[Objective] A HPLC method was established for the determination of limonene in spearmint oil.[Methods] The limonene was quantitatively determined using Absolute methanol as external standard,separated on a C18 column（4.6 mm×250 mm,5 μm） using methanol-water（75∶25,V/V） as mobile phase at a flow rate of 1.0 ml/min.The UV detection wavelength was set at 200 nm and column temperature was 25 ℃ in HPLC.[Results] The results showed that when the content of limonene were among 0.21-1.05 mg/ml,good linear dependence of peak area on concentration of limonene and the correlation coefficient R2 was 0.995,and the average recovery was 98.0%,RSD was 2.6%;after the calculation,the content of limonene in spearmint oil was 24.2%.[Conclusion] The method was simple,rapid and precise for the analysis of limonene in spearmint oil.

关 键 词：留兰香(Mentha spicata L.) 柠檬烯 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]