SP和CGRP在炎症性肠病大鼠结肠中的表达规律  

SP and CGRP in the Occurrence and Development of Inflammatory Bowel Disease

作　　者：张峰[1,2] 袁川评[2] 柳巨雄[2] 曾林[1] 胡娟峰[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]吉林大学,长春130062

出　　处：《中国比较医学杂志》2011年第12期26-30,97,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(No.30871840)

摘　　要：目的通过研究P物质(SP)和降钙素基因相关肽(CGRP)在炎症性肠病(IBD)大鼠结肠中的表达规律来探讨二者在IBD发生发展过程中发挥的作用。方法采用三硝基苯磺酸(TNBS)化学诱导建立IBD大鼠动物模型,应用real-time RT-PCR方法来检测SP和CGRP mRNA在模型不同时期结肠中的表达变化。结果随着炎症性肠病病理变化的加剧(第3、7天),SP和CGRP在结肠组织中的表达水平显著升高;随着疾病的恢复(第21、28天),其表达水平逐渐降低并趋向于正常水平。从总体来看,在IBD大鼠结肠组织中,SP和CGRP mRNA水平的变化与IBD的发生、发展呈正相关。结论神经递质SP和CGRP在IBD炎症和修复中起重要的神经免疫信号传导作用。Aim To explore the effects of substance P （SP） and calcitonin gene-related peptide （CGRP） on the occurrence and development of inflammatory bowel disease （IBD）. Methods The IBD rat model was generated by chemical induction with trinitrobenzene sulfonic acid （TNBS）. The expression of SP and CGRP in the colon tissues was determined by real-time RT-PCR. Results With the development of pathological changes （on days 3, 7） , the expression level of SP and CGRP in the colon was significantly increased, and then followed by a decline and gradual return to normal levels （on days 21, 28）. Generally speaking, the expression of both SP and CGRP was positively related to the occurrence and development of IBD. Conclusions The results of this study revealed that the neurotransmitters, SP and CGRP, play an important role in signal transduction in the inflammation and repair of inflammatory bowel disease.

关 键 词：炎症性肠病 大鼠 SP CGRP 

分 类 号：R33[医药卫生—人体生理学]