大鼠颈部自体静脉移植模型两种吻合方法的比较  

Comparison of Two Types of Vascular Anastomosis in the Establishment of Autologous Cervical Vein Graft Models in Rats

作　　者：毛张凡[1] 徐小惠[2] 黄杰[1] 耿庆[1] 康敢军[1] 吴晓建[1] 

机构地区：[1]武汉大学人民医院胸心外科,湖北武汉430060 [2]华中科技大学同济医院药剂科,湖北武汉430030

出　　处：《中国比较医学杂志》2011年第12期31-33,41,98,共5页Chinese Journal of Comparative Medicine

摘　　要：目的比较间断吻合和连续吻合法建立静脉桥狭窄动物模型的优劣。方法 SD大鼠20只,分成两组(间断吻合组和连续吻合组),取颈外静脉与颈总动脉行端端吻合。术后4周取下静脉桥,观察桥管通畅性,分析新生内膜与中膜的厚度、面积比。结果连续组与间断组相比手术时间更短,出血更少,但桥管通畅率低,两组内膜增生程度没有显著差异。结论连续吻合用时短,出血少,对术者要求更高,较易形成吻合口狭窄。两者造模效果一样。Objective To compare two types of anastomosis in a rat model of intimal hyperplasia of autologous vein graft in rats. Methods Twenty SD rats were randomly divided into two groups. The external jugular veins were anastomosized with the common carotid artery of the same side with interrupted suture and twice continuous suture, respectively. Tissue samples were harvested at 4 weeks after the operation. The vein graft patency was observed and the thickness and area index of the neointima and media were analyzed by histopathology with HE staining. Results Compared with the interrupted suture group, the twice continuous suture showed less time consuming, less bleeding, but also less graft patency. The intimal hyperplasia in the vein grafts occurred after the two anastomosis methods had no significant difference. Conclusions The twice continuous suture has advantages of less time consuming and less bleeding, but it requires more skill. There is no significant difference in the degree of intimal hyperplasia of autologous vein grafts in rats by the two anastomosis methods.

关 键 词：模型 动物 吻合方法 静脉移植物 再狭窄 

分 类 号：R33[医药卫生—人体生理学]