营养不良性肺水肿大鼠肺泡液体清除机制的研究  

Mechanisms Responsible for the Changes in Alveolar Fluid Clearance in Rats with Malnutrition

作　　者：李乃静[1] 李伟[2] 谷秀[3] 李胜岐[3] 何平[4] 

机构地区：[1]中国医科大学附属盛京医院老年病科,辽宁沈阳110004 [2]沈阳药科大学药物分析教研室,辽宁沈阳110016 [3]中国医科大学附属盛京医院呼吸科,辽宁沈阳110004 [4]中国医科大学附属盛京医院老年病科,辽宁沈阳110004

出　　处：《中国比较医学杂志》2011年第12期34-37,F0003,共5页Chinese Journal of Comparative Medicine

摘　　要：目的探讨营养不良性肺水肿大鼠肺泡液体清除功能的变化及其机制。方法制备营养不良性肺水肿大鼠动物模型,分别于48 h和120 h测定大鼠肺泡液体清除率(AFC)、总肺水量(TLW)和肺血管外水量(EVLW)。将钠通道阻断剂氨氯吡咪、Na-K-ATP酶阻断剂哇巴因及β2受体激动剂特布他林分别灌注到正常及禁食120 h大鼠的肺泡腔内,测定AFC的变化。结果大鼠禁食48 h AFC(19.7±3.22%)与正常大鼠AFC(18.5±2.21%)比较没有明显变化;120 h时AFC(9.50±2.19%)明显降低。氨氯吡咪、哇巴因明显降低营养不良性肺水肿大鼠AFC(P<0.05),特布他林对营养不良性肺水肿大鼠AFC的作用与对照组大鼠比较差异无显著性(P>0.05)。结论营养不良性肺水肿与钠通道及Na-K-ATP酶及的活性被抑制,导致肺泡液体清除能力降低有关。Objective To study the mechanisms responsible for the changes in alveolar fluid clearence in rats with malnutrition. Methods Rats were fasted but allowed access to water for 120 hours. Alveolar fluid clearance （AFC） , lung water content （TLW） and extravascular lung water （EVLW） of the rats were calculated in the rats with malnutrition. Isotonic 5% album in solutions with amiloride, ouabain and terbutalin were instilled into the distal airways in the malnutrition and control rat lungs, and the AFC was examined. Results As compared with the control rats （ 18.5 ± 2.21% ） , AFC in the rats with malnutrition for 48 h was not decreased （ 19. 7 ± 3.19% ）. AFC in the rats under conditions of malnutrition for 120 h was decreased （9. 50 ± 2. 19% ）. Amiloride and ouabain could reduce the AFC in both malnutrition and control rats. There was no significant difference in the magnitude of the terbutalin-stimilated AFC in rats under conditions of malnutrition compared with that in control rats. Conclusion Malnutrition primarily impairs amiloridesensitive and amiloride-insensitive alveolar fluid clearance, leading to pulmonary edema.

关 键 词：营养不良 肺泡液体清除率 肺水肿 肺损伤 

分 类 号：R33[医药卫生—人体生理学]