冠脉结扎和介入两种方法制备犬心肌梗死模型的比较  

Comparison of Two Canine Models of Acute Myocardial Infarction Established by Coronary Artery Ligation and Coronary Embolism

作　　者：刘素蕊[1] 赵春生[1] 王更银[1] 李振奇[1] 李俊峡[1] 

机构地区：[1]解放军白求恩国际和平医院,石家庄050082

出　　处：《中国比较医学杂志》2011年第12期38-41,F0003,共5页Chinese Journal of Comparative Medicine

摘　　要：目的观察开胸结扎冠状动脉与闭胸明胶海绵栓塞法制备急性心肌梗死(AMI)动物模型的特点。方法分别经开胸结扎犬冠状动脉左前降支主干及闭胸冠脉栓塞的方法阻断冠脉血流;采用单级肢体导联和胸导联方式,在阻断前后监测心电图波形变化;造模72 h后取心肌组织行病理切片染色。结果经心电图和病理验证,两种方法均可成功制备犬心梗模型,开胸冠脉结扎犬死亡率较高,而冠脉栓塞成活率高。结论相较开胸冠脉结扎法,闭胸栓塞法制备心梗模型对动物损伤小,成活率高,具推广价值。Objective To observe the characteristics of coronary artery ligation and coronary embolism for preparation of acute myocardial infarction （AMI） models in canine. Methods The left main anterior descending coronary artery was obstructed by either open thoracotomy ligation or closed-chest coronary embolism. The electrocardiogram changes were recorded before and after blocking coronary blood flow. At 72 hours after the acute myocardium infarction model preparation, the dogs were sacrificed and specimens of the myocardium were taken for histopathologieal examination. Results Both electrocardiogram and histopathology confirmed that both methods could establish AMI models successfully. Compared with open-chest coronary artery ligation, the dosed-chest coronary embolism had a higher survival rate. Conclusion Closed-chest coronary artery embolism deserves recommendation due to the advantages such as safety and convenience in the preparation of dog models of acute myocardial infarction.

关 键 词：急性心肌梗死 模型 冠脉结扎 犬 

分 类 号：R33[医药卫生—人体生理学]