小型猪腹壁拉链模型的建立  

Establishment of a Miniature Pig Model of Abdominal Wall Biological Zipper

作　　者：马雷[1] 汤球[1] 刘志学[1] 余琛琳[1] 崔淑芳[1] 

机构地区：[1]第二军医大学训练部实验动物中心,上海200433

出　　处：《中国比较医学杂志》2011年第12期61-65,共5页Chinese Journal of Comparative Medicine

基　　金：上海市科技发展基金项目(No.08140901400)

摘　　要：目的建立小型猪腹壁拉链模型并对其生物学特性进行研究,为教学和科研工作的开展提供便利的研究工具。方法通过外科手术的方法将定制的生物拉链固定于小型猪体表,建立小型猪腹壁拉链模型,观察小型猪的体征和精神状态,利用全自动血液分析仪及尿液分析仪对其血液和尿液生化指标进行动态检测。结果模型建立后7～49 d小型猪的活动、精神状态良好,其生理生化指标表现稳定,与对照相比无显著变化。结论本课题组建立的小型猪腹壁拉链模型建立后7～49 d体征、精神状况良好,生理生化指标稳定,可以推广应用于相关的科研、教学试验。Objective To establish a miniature pig model of abdominal wall biological zipper, to study its biological characteristics, and to provide a useful and convenient tool for research and teaching. Methods A selfconstructed special biological zipper was fixed on the abdominal wall of miniature pigs by surgery, and to establish an animal model of abdominal wall zipper. The changes of physiological and biochemical indexes of blood and urine of the miniature pig models were measured at 0, 1, 3, 7, 14, 21, 28, 35, 49, 56 days after surgery and the data were analyzed. The blood physiological and biochemical parameters were RBC, Hb, PLT, total proteins （TP）, albumin （ALB）, total cholesterol （TC） , triglyeeride （TG） , sodium （ Na ~ ） , potassium （ K ~ ） , and chlorine （ C1- ）. The urine parameters included glucose （Glu）, proteins （Pro）, bilirubin （BIL）, urobilinogen （URO）, specific gravity （SG）, pH, leukocytes （WBC）, erythrocytes （RBC）, and nitrite （Nit）. Results The general condition, daily behavior, physiological and biochemical parameters of blood and urine of the miniature pig models showed no significant changes during the 7 -49 day period after modeling, compared with those in the control pigs. Conclusions A miniature pig model of abdominal wall biological zipper has been successfully established in this study, showing stable physiological and biochemical functions during the 7 -49 day period after modeling. This model is suitable for wide application in relevant research and teaching works.

关 键 词：小型猪 腹壁拉链 模型 

分 类 号：R33[医药卫生—人体生理学]