单核苷酸多态性分析在近交系大鼠遗传检测中的应用  

Application of SNP assay in genetic monitoring of inbred rats

作　　者：王淑菁[1] 岳秉飞[1] 马丽颖[1] 王洪[1] 魏杰[1] 刘军须[2] 刘福英[2] 刘双环[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]河北医科大学,石家庄050017

出　　处：《中国实验动物学报》2011年第6期499-504,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：河北省科技支撑计划项目(09276426D)

摘　　要：目的研究SNP在近交系大鼠遗传检测中的应用。方法 选取大鼠20号染色体MHC所在P12区上的9个SNP位点,应用新建立的高保真酶特异性检测SNP基因分型技术对五种常用近交系大鼠(BN、F344、WKY、LEW、SHR)和两种新培育近交系大鼠(MIJ和HFJ)进行SNP多态性分析。结果五种常用近交系的SNP检测结果与Rat Genome Database网站提供的基因型数据一致,并检测确立了新品系的SNP基因型。同时绘制出七种近交系大鼠在该9个SNP位点的遗传扩增图谱。结论运用所筛选的9个SNP位点进行大鼠多态性分析,能够快速、可靠地对BN、F344、WKY、LEW、SHR及MIJ、HFJ进行遗传监测。Objective To investigate the feasibility of single nucleotide polymorphism（SNP）analysis in genetic monitoring of inbred rats.Methods Nine SNP loci in rat major histocompatibility complex（RT1） of the 20th chromosome were investigated.Pfu DNA polymerase using phosphorothioate-modified primers specifically amplified the genome template of 7 types of rat inbreds.Results The genotype results of 5 inbred rats（BN,F344,WKY,LEW,SHR） was consistent with the data from Rat Genome Database website,and the genotypes of newly bred inbreds（MIJ,HFJ） were detected.Conclusions The panel of 9 SNP loci polymorphism analysis is a rapid and reliable way for genetic monitoring inbred rats（BN,F344,WKY,LEW,SHR,MIJ and HFJ）.

关 键 词：单核苷酸多态性(SNP) 高保真酶 近交系大鼠 遗传检测 

分 类 号：Q95-33[生物学—动物学]