雌激素对人乳腺癌细胞周期蛋白E表达及血清饥饿时细胞存活力的影响  

Effects of Estrogen on the Expression of Cyclin E and Cell Viability during Serum Starvation in Human Breast Cancer Cells

作　　者：刘晓红[1] 易亮[2] 陈冷昕[2] 陈腾祥[1] 王旭东[1] 

机构地区：[1]贵阳医学院生理学教研室,贵州贵阳550004 [2]贵阳医学院

出　　处：《贵阳医学院学报》2011年第6期551-553,557,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(30860093)

摘　　要：目的:探讨雌激素对人乳腺癌细胞周期蛋白E表达及在血清饥饿状态下细胞存活力的影响。方法:以MCF-7乳腺癌细胞为模型,采用蛋白印迹分析法检测乳腺癌周期蛋白与表达、显微镜下观察细胞变化、台盼蓝染色及细胞计数法测算细胞存活力。结果:雌激素(10 nmol/L)处理可引起乳腺癌细胞周期蛋白E的过度表达,与对照组比较,增高56.42%(P<0.05);在无血清培养条件下,雌激素能减少乳腺癌细胞死亡,增强细胞的存活力,与对照组比较,72 h细胞存活率增高19.86%(P<0.05)。结论:雌激素能显著上调周期蛋白E的蛋白表达,并增强乳腺癌细胞对血清饥饿的抵抗力。Objective： To explore the effects of estrogen on the expression of cyclin E and cell viability during serum starvation in human breast cancer cells.Methods： MCF-7 human breast cancer cells were employed as study model.Western blotting was used to detect the expression of cyclin E protein.Changes of cell morphology were observed under microscope.Trypan Blue staining and cell counting were employed to determine cell viability.Results： In breast cancer cells,estrogen（10 nmol/L） could increase the expression of cyclin E by 56.42% as compared with that of control（P0.02）.On the condition of serum-free culture,estrogen reduced cell death and increased cell viability.With the action of estrogen,cell viability increased gradually with the time passing.Cell viability increased by 19.86% in 72 h as compared with that of control（P0.05）.Conclusions： Estrogen contributes to increased expression of cyclin E and enhances resistance of breast cancer cells to serum starvation.

关 键 词：乳腺肿瘤 雌激素类 细胞周期蛋白E 细胞存活 

分 类 号：R373.9[医药卫生—病原生物学]