CD44在重症急性胰腺炎大鼠肝脏表达及清胰Ⅱ号颗粒剂保护作用的实验研究  

Expression of CD44 in Liver Tissues of Rats with Severe Acute Pancreatitis and Protective Effect of Qingyi Ⅱ Granules

作　　者：李鹏[1] 王玲君[2] 兑丹华[3] 

机构地区：[1]滨州医学院附院肛肠外科,山东滨州256603 [2]博兴县人民医院,山东博兴256500 [3]遵义医学院附院肝胆胰外科,贵州遵义563003

出　　处：《贵阳医学院学报》2011年第6期561-564,共4页Journal of Guiyang Medical College

基　　金：贵州省科技厅中医药现代化资助项目[2003(13)]

摘　　要：目的:探讨CD44在重症急性胰腺炎(severe acute pancreastitis,SAP)时胰腺及肝损害机制中的作用,并观察中药清胰Ⅱ号颗粒剂对CD44的影响及机理。方法:将36只SD大鼠随机等分为假手术组(A组)、SAP组(B组)、治疗组(C组),各组以30 g/L牛磺胆酸钠逆行注入胰胆管制作大鼠SAP模型,A组大鼠开腹后仅翻动肠管后关腹,术后1 h C组以250 g/L清胰Ⅱ号灌胃给药(10 mL/kg)6 h 1次,A组、B组用同等剂量生理盐水以相同方式灌胃;制模后24 h取材,抽血测定血清淀粉酶(amylase,AMY)、谷草转氨酶(Aspartate amin-otransferase,AST)、谷丙转氨酶(Alanine aminotransferase,ALT)活性的变化,HE染色观察胰腺、肝脏组织病理改变,应用免疫组织化方法检测胰腺、肝组织中CD44的表达。结果:SAP组胰腺及肝脏组织损伤明显,CD44的表达呈阳性,血清AMY、AST、ALT活性显著升高,与A组、C组比较有统计学意义(P<0.05);C组胰腺及肝脏组织中CD44的表达减弱,血清AMY、AST、ALT活性降低,与SAP组比较有统计学意义(P<0.05)。结论:CD44在SAP模型大鼠肝损伤中可能具有重要作用,中药清胰Ⅱ号颗粒可抑制胰腺、肝脏组织CD44的表达,减轻SAP时胰腺和肝脏损害。Objective： To explore the role of CD44 in pancreas and liver damage in severe acute pancreastitis（SAP）,and to investigate the effects of Chinese medicine Qingyi Ⅱ granules on the expression of CD44 and its mechanism.Methods： A total of 36 healthy SD rats were randomly divided into three groups： sham-operated group（group A）,SAP model group（group B） and treatment group（group C）.SAP model was established by retrograde injection of 30 g/L sodium taurocholate into bile-pancreas duct.Qingyi Ⅱ Granules 250 g/L was administered by gavage every 6 hours in group C.While the other two groups were infused with same volume of saline instead.All animals were sacrificed after modeling for 24 h.The CD44 expressions in pancreas and liver tissues were detected with immunohistochemistry method.Serum amylase（AMY）,aspartate aminotransferasea（AST） and alanine aminotransferase（ALT） were tested.Pathologic changes of pancreas and liver tissues were observed under microscope.Results： Pancreas and liver tissues were damaged seriously in group B,and the expression of CD44 was positive,the serum levels of AMY,AST and ALT were significantly higher than those of groups A and C（P0.05）.The expression level of CD44 was lower and activities of serum AMY,AST and ALT were higher significantly in group C than those in group B.（P0.05）.Conclusions： The expression of CD44 may play an important role in pancreas and liver injury in SAP rats.Chinese medicine Qingyi Ⅱ granules could depress the expressions of CD44 in pancreas and liver tissues,and alleviate the pathologic damage of pancreas and liver in SAP rats.

关 键 词：抗原 CD44 胰腺炎 免疫组织化学 大鼠 

分 类 号：R657.51[医药卫生—外科学] R361.1[医药卫生—临床医学]