半胱氨酸蛋白酶抑制剂C对自发性高血压大鼠心肌纤维化的影响  

Effect of cystatin C on myocardial fibrosis in spontaneously hypertensive rats

作　　者：闫莉[1,2] 巩会萍[1] 杜贻萌[1] 王欣[1] 徐冬玲[1] 魏峰涛[1] 王旭平[2] 鹿庆华[1] 

机构地区：[1]山东大学第二医院心内科,济南250033 [2]山东大学齐鲁医院教育部与卫生部心血管重构和功能研究重点实验室,济南250012

出　　处：《山东大学学报（医学版）》2011年第12期42-47,共6页Journal of Shandong University:Health Sciences

基　　金：山东省科学技术发展计划资助项目(2007GG20002028)

摘　　要：目的探讨半胱氨酸蛋白酶抑制剂C(cystatin C)对于自发性高血压大鼠(SHR)心肌纤维化的影响。方法培养16周龄的自发性高血压大鼠心肌成纤维细胞(CFs),将构建成功含cystatin C的腺病毒载体转染SHR的CFs及心室肌中,随机分为空白对照组、Ad-GFP组和Ad-CysC组。通过w estern blot检测cystatin C在CFs和SHR心室肌中的表达情况。用实时定量PCR测定CFs的Ⅰ、Ⅲ胶原mRNA水平。行超声心动检查评定转染后4周SHR的心脏结构和心功能。通过Masson染色分析SHR的心肌纤维化程度。结果 Cystatin C在Ad-CysC组CFs和心室肌中表达较空白对照组和Ad-GFP组明显增强(P均<0.05);与空白对照组和Ad-GFP组相比,Ad-CysC组CFs中Ⅰ、Ⅲ胶原mRNA表达显著增加(P均<0.05);Ad-CysC组LVMI、IVSD、LVPWD、CVF和PVCA明显高于空白对照组和Ad-GFP组,LVEDD较其余两组明显减小(P均<0.05)。上述指标在空白对照组和Ad-GFP组中差异均无统计学意义(P>0.05)。结论 Cystatin C使SHR成纤维细胞胶原合成增多,具有促进心肌纤维化进程的作用。Objective To investigate the effect of cystatin C on myocardial fibrosis in spontaneously hypertensive rats（SHR）.Methods Cardiac fibroblasts（CFs） of SHR aged 16 weeks were cultured in vitro.The recombinant adenovirus vector for cystatin C was transfected into CFs and the ventricular myocardium.All rats were randomly divided into 3 groups： the normal control group,the Ad-GFP group and the Ad-CysC group.Expression of cystatin C in CFs and the ventricular myocardium was determined by means of Western blot.MRNA expressions of collagen Ⅰ and Ⅲ were determined by RT-PCR.Echocardiography was used to evaluate the cardiac structure and function after transfection for 4 weeks.Myocardial fibrosis was analyzed with Masson′s staining.Results Protein expression of cystatin C was increased in CFs and the ventricle myocardium in the Ad-CysC group when compared with the normal control group and the Ad-GFP group（both P0.05）.Compared with the normal control group and the Ad-GFP group,mRNA expressions of collagenⅠ and Ⅲ were increased in the Ad-CysC group（both P0.05）;The LVMI,IVSD,LVPWD,CVF and PVCA were more increased and the LVEDD was more decreased in the Ad-CysC group than the normal control group and the Ad-GFP group（all P0.05）,while there was no statistical significance between the Ad-GFP group and the normal control group（P0.05）.Conclusion Cystatin C promotes collagen synthesis in CFs and may play a role in the progression of myocardial fibrosis in SHR.

关 键 词：半胱氨酸蛋白酶抑制剂C 自发性高血压大鼠 心肌成纤维细胞 Ⅰ Ⅲ胶原蛋白 

分 类 号：R541.3[医药卫生—心血管疾病]