人乳头瘤病毒16型E7蛋白单克隆抗体的研制  

Preparation of a monoclonal antibody against the human papillomavirus type 16 E7(HPV 16 E7) protein

作　　者：阴秀丽[1,2] 李坤[3] 宁涛 徐昌青[3] 陈自平[3] 

机构地区：[1]山东大学医学院,济南250012 [2]山东省荣军总医院消化内科,济南250013 [3]山东大学附属千佛山医院消化内科,济南250014 [4]北京市肿瘤防治研究所遗传室,北京100142

出　　处：《山东大学学报（医学版）》2011年第12期62-65,共4页Journal of Shandong University:Health Sciences

基　　金：山东省科技攻关计划资助项目(2006GG3202017)

摘　　要：目的为研究HPV16 E7蛋白在肿瘤早期预报中的作用及研制治疗性疫苗,特制备HPV16 E7的单克隆抗体。方法将构建成的PGEX4T-1-HPV16 E7的质粒转化BL21细菌,在IPTG诱导下,稳定表达GST-HPV16 E7融合蛋白,免疫Balb/c小鼠,收集免疫小鼠的脾细胞与SP2/0细胞融合,用ELISA双筛,直至检测所有孔均为E7阳性而GST阴性为止,扩大培养、建株、冻存并制备腹水、单克隆抗体。结果成功制备出HPV16 E7的单克隆抗体,可在多种肿瘤组织细胞核中染色。结论该抗体是HPV16 E7蛋白特异性的McAb。Objective An HPV 16 E7 monoclonal antibody was prepared.This antibody will be useful for diagnosing early cancers and developing therapeutic vaccines.Method A plasmid constructed with PGEX4T-1-HPV16 E7 was transformed into E.coli BL21,and expression of the fusion protein was induced by IPTG.Spleen cells from immunized Balb/c mice were fused with SP2/0 cells by a routine method,and the valence of the antibody was evaluated using ELISA.Expansion,isolation and freezing of clones were carried out,and finally an ascitic fluid which contained monoclonal antibodies was produced.Result The HPV16 E7 monoclonal antibody was found specifically in the nuclei of a variety of cancers tissues.Conclusion The HPV16 E7 protein antibody is a specific McAb.

关 键 词：消化道肿瘤 人乳头瘤病毒16E7 单克隆抗体 

分 类 号：R373.9[医药卫生—病原生物学]