吸入性糖皮质激素及白三烯受体拮抗剂对哮喘小鼠肺组织IL-6表达的影响  

Effect of inhaled glucocorticoid and leukotriene receptor on IL-6 expression in the lungs of asthmatic mice

作　　者：尤海章[1] 周卫芳[1] 季伟[1] 刘芬菊[2] 陈秋[2] 金美芳[1] 孙惠泉[1] 张明芝[2] 

机构地区：[1]苏州大学附属儿童医院呼吸病科,215003 [2]苏州大学放射与公共卫生学院

出　　处：《江苏医药》2011年第24期2893-2895,F0002,共4页Jiangsu Medical Journal

基　　金：苏州市科技发展计划项目(SYS201142)

摘　　要：目的探讨白细胞介素6(IL-6)在小鼠哮喘模型肺组织表达及吸入性糖皮质激素(ICS)、白三烯受体拮抗剂对其影响。方法 96只雄性Balb/c小鼠随机分为哮喘组、布地奈德组、孟鲁斯特组和对照组,每组24只。每组又分为第1周、第2周、第3周、第4周4个亚组,每亚组6只。免疫组化标记IL-6在肺组织的分布,Image-Pro Plus图像分析系统分析IL-6的灰度值。结果成功制作哮喘小鼠模型。与对照组相比,哮喘组、布地奈德组、孟鲁斯特组IL-6表达在第1～4周时差异有统计学意义(P<0.05);孟鲁斯特组IL-6表达在第3周时较布地奈德组增多(P<0.05);哮喘组、布地奈德组、孟鲁斯特组在不同时间点组内差异均有统计学意义(P<0.05)。结论 IL-6参与哮喘气道炎症。布地奈德、孟鲁斯特可减少哮喘小鼠肺组织IL-6的表达,布地奈德作用优于孟鲁斯特。Objective To investigate the effect of inhaled glucocorticoid and leukotriene receptor antagonist on the interleukin-6（IL-6） expression in the lungs of asthmatic mice.MethodsNinety-six male Balb/c mice were randomly divided into four groups of A（asthmatic mice group）,B（budesonide group）,C（montelukast group） and D（control group） with 24 mice each.Six mice in each group were sacrificed to detect IL-6 expression at the end of the 1st,2nd,3rd and 4th week,respectively.The distribution of IL-6 expression was marked with immunohistochemistry.The IL-6 grey value was analyzed by Image-Pro Plus analysis system.Results The asthmatic mice model was successfully established.Compared with group D,the IL-6 expression was significantly different in groups of A,B and C at different time points（P0.05）,which was higher in group C than that in group B at the end of the 3rd week（P0.05）.There were significant differences of IL-6 expressions among different time points in groups of A,B and C,respectively（P0.05）.Conclusion IL-6 plays a role in airway inflammation of asthma.Budesonide is able to decrease the IL-6 expression in lung tissues of asthmatic mice,which of budesonide is stronger than that of montelukast.

关 键 词：糖皮质激素 白三烯受体拮抗剂 白细胞介素6 哮喘 

分 类 号：R562[医药卫生—呼吸系统]