南极假单胞菌Pseudomonas sp．P4—11对黄瓜白粉病的防效和毒理学试验  

Biocontrol Effect on Cucumber Powdery Mildew and Toxicity Test of Antarctic Pseudomonas sp. P4-11

作　　者：王红梅[1,2,3] 何培青[1,2] 林学政[1,2] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]国家海洋局海洋生物活性物质重点实验室,山东青岛266061 [3]山东轻工业学院食品与生物工程学院,山东济南250353

出　　处：《海洋科学进展》2011年第A01期87-94,共8页Advances in Marine Science

基　　金：国家高技术研究发展计划项目极地特殊海洋微生物资源利用的关键技术研究（2007AA091905）;海洋公益性行业科研专项经费项目--深远海（极地）微生物及其基因资源开发与产品应用示范（2010050322）

摘　　要：从南极海水中获得一株抗植物病原真菌活性菌株P4—11，利用16SrRNA技术对其进行了分子鉴定，测定了菌株对大棚黄瓜白粉病的防治效果，并进行了大鼠急性经皮、急性经口以及孔雀鱼的延长毒性试验。结果表明，活性菌株P4—11属于假单胞菌属（Pseudomonas）。生防试验表明，菌株P411发酵液的1：1稀释液对黄瓜白粉病的防治效果为68．26％，1：2稀释液的防治效果为66．57％，均高于1％武夷菌素水剂500倍稀释液63．71％的防效。毒理学试验结果表明，菌株P4—11发酵液的大鼠急性经皮、急性经口毒性等级为低毒，对孔雀鱼未见异常。以上实验结果表明，菌株P4-11在植物病害的生物防治中具有潜在的应用价值。An Antarctic bacterium P4-11 was isolated from Antarctic seawater. Its molecular identification was determined using 16S rRNA sequence analysis, its bioeontrol effect on greenhouse cucumber powdery mildew was determined and both acute dermal test and oral toxicities on the guppy were also studied. The results show that the strain P4-11 belongs to Pseudomonas species. Biocontrol effect on cucumber powdery mildew was 68.26 % with the fermentation broth dilution 1：1, and the control effect was 66.57% with 1 ：2 dilution, higher than that of 63. 71; with 1% Wuyi streptozotocin 500-fold dilution. Toxicity tests showed the level of acute dermal toxicity and acute oral toxicity of strain P4-11 fermentation broth was low, no abnormalities were detected on guppy. The strain has potential applications in biological control of plant diseases.

关 键 词：南极 假单胞菌P4—11 黄瓜自粉病 毒理试验 

分 类 号：S432.4[农业科学—植物病理学]