高效液相色谱-二极管阵列法检测复方川羚定喘胶囊中添加的茶碱  

Determination of theophylline by HPLC-DAD in compounded Chuanling Dingchuan capsules

作　　者：董志华[1] 陈乃江[2] 

机构地区：[1]连云港市第二人民医院,江苏连云港222023 [2]连云港市药品检验所,江苏连云港222006

出　　处：《中国新药与临床杂志》2011年第12期950-952,共3页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的建立检测复方川羚定喘胶囊中添加茶碱的专属性方法。方法采用高效液相色谱一二极管阵列检测器(HPLC-DAD)检测,选用Phenomenex C_(18) Gemini(250 mm×4.6 mm,5μm)色谱柱,以0.01 mol·L^(-1)磷酸氢二钾溶液:乙腈(70:30,V/V)为流动相,检测波长:DAD 200～400nm。通过与对照品的色谱、紫外光谱行为相比较,对此类非法添加茶碱的复方川羚定喘胶囊制剂进行定量测定和定性鉴别。结果对照品和检测样品在紫外色谱检测中,DAD 272.2 nm处均出现茶碱最大吸收峰,茶碱对照品平均回收率为(97.5±1.1)%(n=6),每粒检测样品茶碱含量为(80.0±1.9)mg(n=3),结果显示检测样品中非法加入了茶碱。结论该方法选择性强,灵敏度高,可作为分析检测此类制剂中非法加入的茶碱。AIM To study the antiarrhythmic effect of sophocarpine and its mechanism.METHODS A rapid cardiac arrhythmic SD rat model induced by aconitune was used,dividing into 3 groups such as model group,sophocarpine group（5 mg·kg^（-1）,iv） and amiodarone group（2.5μg·kg^（-1）,iv）.Continuous EKG monitoring and printing were applied to keep track of the appearing time of ventricular premature beat, ventricular tachycardia,ventricular flutter,ventricular fibrillation and cardiac arrest,which could be changed into total consumption dose of aconitine so as to be compared among groups,then the antiarrhythmia effect of sophocarpine could be evaluated.Capillary grass microelectrode was used to record action potential of guinea pig papillary muscles in vitro.Sophocarpine of different concentrations（1、5、10μmol·L^（-1）） were added to the perfusion sulotion separately in order to investigate how they would affect action potential duration（APD）, effective refractory period（ERP）,maximal depolarization velocity（V_（max））,action potential amplitude（APA） and resting potential（RP）.RESULTS The appearance of ventricular premature beat,ventricular tachycardia, ventricular flutter,ventricular fibrillation and cardiac arrest were all delayed in the sophocarpine group and the amiodarone group in comparison with the model group（P0.05）,but there was no significant differences between the sophocarpine group and the amiodarone group（P0.05）.Sophocarpine（1、5、10μmol·L^（-1）） prolonged APD_（50）,APD_（90）,APD_（100） and ERP while V_（max） declined（P0.05）,but it had little influence on APA and RP（P0.05）.CONCLUSION Sophocarpine has significant antiarrhythmic effect on the aconitine induced arrhythmia rat mode,which is possibly due to prolongation of APD and ERP.

关 键 词：茶碱 色谱法 高压液相 药物污染 复方川羚定喘胶囊 

分 类 号：R927.11[医药卫生—药学]