陆地棉干旱胁迫相关基因GhTrx的克隆及表达  

Cloning and Expression of Drought-stress Related Gene GhTrx in Gossypium hirsutum L.

作　　者：宋贵方[1,2] 周凯[1] 王俊娟[1] 樊伟丽[1] 王德龙[1] 王帅[1] 叶武威[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室/农业部棉花遗传改良重点开放实验室,河南安阳455000 [2]河南大学生命科学学院,河南开封475000

出　　处：《西北植物学报》2011年第10期1951-1957,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：973计划(2010CB126006);转基因生物新品种培育科技重大专项(2008ZX08005-004);"十一五"国家科技支撑计划(2006BAD13B04-1)

摘　　要：采用RACE和RT-PCR技术,克隆了陆地棉干旱胁迫硫氧还蛋白基因,利用荧光定量PCR技术分析该基因在不同组织和不同时期的表达情况.结果表明,陆地棉硫氧还蛋白基因(GhTrx)的cDNA全长1 340 bp,其中ORF 864 bp,推测编码288个氨基酸.该基因编码蛋白与杨树、蓖麻、拟南芥的相似性分别为70%、72%和76%,系统发育树分析显示,GhTrx与蓖麻中该蛋白的亲缘关系最近.RT-PCR分析显示,GhTrx基因表达受干旱胁迫诱导,在干旱诱导下,该基因在抗旱材料中H177中上调表达,在根中的表达量明显高于叶.研究表明,GhTrx基因在干旱胁迫时根部进行大量表达,对抵御外界的干旱威胁起到关键作用,可能对提高陆地棉抗旱性方面具有一定的作用.In this research,thioredoxin gene from Gossypium hirsutum L.,named GhTrx,was initially cloned by RACE and RT-PCR techniques.The cDNA full length was 1 340 bp,and ORF is 864 bp,which encoded 288 amino acid residues.Its express condition was analyzed by real-time PCR at different organizations and different periods GhTrx had the similarity of 70%,72% and 76% to Populus trichocarpa,Ricinus communis and Arabidopsis thaliana corresponding gene,respectively.Phylogenetic analysis showed GhTrx was the closest to Ricinus communis,and real-time PCR suggested that GhTrx protein could be induced by drought tolerance.With drought stress,GhTrx showed high expression level on drought-stress materials,＇Zhong H177＇,especially outstanding higher expression in root than in leaf.Thus,we speculate that GhTrx gene increases of its expression on drought-stress condition in root against outside drought environment.It may plays a role in improving drought resistance capacity of Gossypium hirsutum L.

关 键 词：陆地棉 棉花硫氧还蛋白基因 干旱胁迫 上调表达 

分 类 号：Q785[生物学—分子生物学] Q786