3种地被类观赏竹对大气臭氧浓度倍增的生理响应  

Physiological Responses of Three Dwarf Ornamental Bamboos to the Elevated Atmospheric Ozone Concentration

作　　者：庄明浩[1] 李迎春[1] 李应[1,2] 郭子武[1] 杨清平[1] 顾大形[1] 陈双林[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]贵州大学林学院,贵州贵阳550025

出　　处：《西北植物学报》2011年第10期2014-2020,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金(RISF6915)

摘　　要：运用开顶式同化箱（OTCs）模拟环境背景大气O3浓度（CK,40~45 nL.L-1）的倍增1倍（TR-1,92~106nL.L-1）和倍增2倍（TR-2,142~160 nL.L-1）情景,分析美丽箬竹、黄条金刚竹和白缟椎谷笹竹叶片脂质过氧化、抗氧化酶和渗透调节物质等生理指标的变化,以明确竹子对大气O3浓度倍增的生理响应规律及耐受O3胁迫能力的种间差异性,为园林观赏竹种选育和应用提供参考。结果表明：（1）大气O3浓度倍增45 d时,随着O3浓度的升高,3种地被类观赏竹叶片O2.-和MDA含量、相对电导率、SOD活性均表现出增大趋势。叶片POD活性、可溶性糖含量美丽箬竹呈下降趋势,而黄条金刚竹和白缟椎谷笹竹呈升高趋势。叶片可溶性蛋白含量美丽箬竹和白缟椎谷笹竹呈下降趋势,黄条金刚竹呈升高趋势。（2）大气O3浓度倍增1倍时,美丽箬竹和黄条金刚竹未表现出伤害症状,而白缟椎谷笹竹表现出明显的伤害症状;大气O3浓度倍增2倍时,3种地被类观赏竹叶片活性氧含量大量积累,抗氧化系统遭到破坏,膜脂过氧化加剧,影响正常的生理代谢和生长发育。（3）主成分分析表明,叶片MDA含量是反映地被类观赏竹耐受O3胁迫最重要的生理指标,耐受O3胁迫能力表现为美丽箬竹〉黄条金刚竹〉白缟椎谷笹竹。In order to probe the physiological responses of bamboos on O3 concentrations rising and interspecific difference of the tolerance to O3 under the background of climatic change,meanwhile to provide theoretical evidences for bamboo cultivation,the method of open-top chambers（OTCs） was employed to approach the effects of elevated O3 on lipid peroxidation,anti-oxidation enzyme system and osmotic adjusting substances in the leaves of three dwarf ornamental bamboos.The results indicated that：（1）After 45 d,with the ozone concentration increasing,O-·2 content and MDA content and relative electron conduction and SOD activity increased in the three ornamental bamboos.The POD activity and soluble sugar content decreased in the leaves of Indocalamus decorus,while increased in the leaves of Pleioblastus kongosanensis and Sasa glabra.The soluble protein content decreased in the leaves of I.decorus and S.glabra,however,increased in the leaves of P.kongosanensis.（2）In the treatment TR-1,I.decorus and P.kongosanensis did not show injury,but S.glabra showed injury significantly.In the treatment of TR-2 lead reactive oxygen accumulation in leaves of three dwarf ornamental bamboos,intensified the damage to membrane lipid,and the function of anti-oxidation system as well,consequently,the growth of three dwarf ornamental bamboos would be affected.（3）Principal component analysis showed that MDA content was the most important indicator which provided a quantitative evaluation of the different physiological indices for dwarf ornamental bamboos under the ozone stress,which indicated that the order of the adapability and the tolerance to ozone of the three ornamental bamboos was I.decorusP.kongosanensisS.glabra.

关 键 词：地被类观赏竹 臭氧胁迫 膜脂过氧化 抗氧化酶 渗透调节物质 

分 类 号：Q945.79[生物学—植物学]