响应面法优化球孢白僵菌CXJ-1的发酵工艺条件  

Optimizatiom of Fermentation Conditons for Beauveria bassiana CXJ-1Spore by Response Surface Methodology

作　　者：孙佰平[1,2] 任娟[2] 赵思峰[2] 侯彩霞[2] 王斌[2] 刘启鹏[2] 

机构地区：[1]新疆兵团化工绿色过程重点实验室,石河子832003 [2]石河子大学绿洲农作物病害防控重点实验室,石河子832003

出　　处：《石河子大学学报（自然科学版）》2011年第6期689-695,共7页Journal of Shihezi University(Natural Science)

基　　金：新疆兵团重大产学研专项(2010ZX03-1);国家科技支撑计划项目(2011BAD48B02)

摘　　要：为了提高球孢白僵菌CXJ-1(Beauveria bassiana CXJ-1)在液体发酵过程中分生孢子的产孢量,采用响应面法对球孢白僵菌CXJ-1产孢的发酵工艺条件进行优化。采用单因素实验确定了产孢发酵条件最适初始pH、发酵转速和发酵温度。通过Plackett-Burman实验设计和Box-Behnken实验设计响应面分析优化,确定了球孢白僵菌CXJ-1产孢最佳发酵工艺参数为:温度28.07℃,转速198.95r/min,初始pH 6.10,预测孢子浓度可达9.5×108个/mL,在优化发酵条件下进行验证实验,孢子浓度达到9.7×108个/mL,表明验证值和预测值有较高的吻合度。Response surface methodology was used to optimize fermentation conditons in order to improve spore production of Beauveria bassiana CXJ-1.The optimum fermentation temperature,rotation speed and pH were obtained with one-factor-at-time.Through Plackett-Burman experimental design and Box-Behnken experimental design for response surface optimization,the optimal conditions for spore production of Beauveria bassiana CXJ-1 were also determined as follows：temperature 28.07 ℃,rotation speed 198.95 r/min and the pH value 6.10.Under these conditions,the predicted value of the concentration of spores was 9.5×108 spores/mL and the verified value was 9.7×108 spores/mL,the fermentation validation showed that the experimental value have high inosculating degree to model prediction.

关 键 词：球孢白僵菌 响应面优化 发酵工艺条件 产孢 

分 类 号：Q815[生物学—生物工程]