松材线虫近交系的培育  

Inbreeding of the Pine Wood Nematode,Bursaphelenchus xylophilus

作　　者：朱丽华[1] 应晨希[1] 叶建仁[1] 

机构地区：[1]南京林业大学森林资源与环境学院江苏省有害生物入侵预防与控制重点实验室,南京210037

出　　处：《林业科学》2011年第12期92-96,共5页Scientia Silvae Sinicae

基　　金：国家林业局公益性行业科研专项(200704026);国家自然科学基金项目(30972369);江苏省有害生物入侵预防与控制重点实验室开放基金项目(PMIS200802);江苏高校优势学科建设工程资助项目

摘　　要：松材线虫种群间及种群内存在高度的遗传多样性。为了增加其基因的纯合性,以强毒松材线虫虫株AMA3为原始种群,采用连续全同胞兄妹交配培养20代的方式进行近交系培育,共获得来自3对不同亲本的80个近交系。以感病黑松为宿主比较AMA3原始种群及不同近交种群的致病力。结果表明,连续近交未对松材线虫的致病力造成显著影响,来自3对亲本的F6,F10,F15及F20松材线虫种群依然保持强致病力。The pine wood nematode is a plant parasitic nematode that causes devastating pine-wilt disease.Previous studies have indicated a high degree of genetic heterogeneity between isolates and within isolate.To increase homozygosity,the pine wood nematode was inbred by sibling matings at each generation for 20 generations.Total of 80 inbred lines which from 3 parents of virulent isolate AMA3 were created.The virulence potential of inbred lines in different generation was estimated by inoculation test using susceptible Pinus thunbergii seedlings and then by comparing the resulting host mortality with the original nematode isolate.As a result,the successive inbreeding did not significantly changed the virulence,and inbred lines in 6,10,15 and 20 generation were the same in virulence compared to the original isolates.

关 键 词：松材线虫 全同胞 近交 致病力 

分 类 号：S763.1[农业科学—森林保护学]