应用多种STR技术对基因嵌合体进行亲权鉴定分析  

Paternity Analysis of Gene Chimera with Multiple STR Loci

作　　者：林源[1] 赵珍敏[1] 李莉[1] 

机构地区：[1]司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063

出　　处：《中国司法鉴定》2011年第6期35-37,53,共4页Chinese Journal of Forensic Sciences

基　　金：中央级科研院所社会公益研究资助项目(GY0803)

摘　　要：目的查找嵌合基因的来源并进行父母和孩子的亲权鉴定。方法采用Chelex-100法抽提基因组DNA,用复合扩增和荧光检测技术对STR、X-STR和Y-STR基因座进行分型。结果父亲为XX,XY基因嵌合体,其能够提供给孩子必须的遗传基因。结论父亲为被检孩子的生物学父亲。Objective To detect the source of the chimeric genes and perform paternity test with STR,X-STR and Y-STR techniques.Methods Genomic DNA was extracted by Chelex-100 method.STR,X-STR and Y-STR loci were typed by multiplex amplification and fluorescence detection.Results The gender gene of the detected father is the chimera of XX and XY.The detected father can provide the paternal gene to the detected child.Conclusion The detected father is the child＇s biological father.

关 键 词：PCR复合扩增 荧光检测 STR X-STR Y-STR检测技术 基因嵌合体 

分 类 号：D919.6[医药卫生—法医学]