补充白藜芦醇对大强度运动大鼠脑组织抗氧化能力、NO、NOS、ATP酶的影响  

Effects of resveratrol on anti-oxidation,NO,NOS,ATPase in brain of intensive exercise rats

作　　者：武胜奇[1] 张琳[2] 熊正英[3] 郝选明[2] 

机构地区：[1]广东外语艺术职业学院基础部,广东广州510640 [2]华南师范大学体育科学学院,广东广州510631 [3]陕西师范大学体育学院,陕西西安710062

出　　处：《武汉体育学院学报》2011年第12期78-81,共4页Journal of Wuhan Sports University

基　　金：陕西省自然科学基金资助项目(2003C137)

摘　　要：目的:通过检测补充白藜芦醇(RES)对大强度运动大鼠脑组织抗氧化能力、NO、NOS、ATP酶的影响,探讨RES减缓运动性损伤的效果和机制。方法:以雄性SD大鼠为研究对象,建立大强度递增负荷运动模型,各组大鼠训练结束后匀浆法提取大鼠脑组织匀浆,紫外分光光度计检测大鼠脑组织中总超氧化物歧化酶(tSOD)、CuZn超氧化物歧化酶(CuZn-SOD)、Mn超氧化物歧化酶(Mn-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、丙二醛(MDA)和总一氧化氮合酶(tNOS)、诱导型一氧化氮合酶(i-NOS)、结构型一氧化氮合酶(cNOS)、一氧化氮(NO)以及Na+,K+-ATP酶(Na+,K+-ATPase)、Ca2+-ATP酶(Ca2+-ATPase)的表达水平。结果:大强度运动可造成大鼠脑组织中的tSOD、CuZn-SOD、Mn-SOD、MDA、tNOS、iNOS、NO显著升高,GSH-Px、cNOS、Na+,K+-ATPase、Ca2+-ATPase显著下降。补充RES可使大鼠运动后脑组织中MDA、tNOS、iNOS、NO下降,tSOD、CuZn-SOD、Mn-SOD、GSH-Px、CAT、cNOS、Na+,K+-ATPase、Ca2+-ATPase活性升高。结论:大强度运动造成脑组织氧化损伤,RES能够有效提高脑组织抗氧化能力,保持脑组织ATPase活性,减轻氧化性损伤。This study was to investigate the role of resveratrol to delay exercise-induced injury by detection effects of resveratrol on anti-oxidation, NO, NOS, ATPase in the brain of intensive exercise rats. The exercise- trained male SD rats models were set up by progressing intensive endurance treadmill training. Then we researched into the indices of total superoxide dismutase （tSOD）, CuZn-superoxide dismutase （CuZn-SOD）, Mn- superoxide dismutase（Mn-SOD）, glutathione peroxidase （GSH-Px）, catalase（CAT）, malondialdehyde（MDA）, total nitric oxide synthase （tNOS）, inducible nitric oxide synthase （iNOS）, constitutive nitric oxide synthase （cNOS）, nitric oxide （NO）, Na^＋,K^＋-ATPase, and Ca^2＋-ATPase in brain after every training period. The levels of total SOD, CuZn-SOD, MDA, total NOS, iNOS, and NO in brain increased significantly in rats after intensive exercise; while those of GSH-Px, cNOS, Na^＋,K^＋-ATPase, Ca^2＋-ATPase decreased significantly in rats after intensive exercise. The supplement of resveratrol degraded MDA, total NOS, iNOS, NO in brain after intensive exercise ; while those of total SOD, CuZn-SOD, GSH-Px, cNOS, Na^＋K^＋-ATPase, Ca^2＋-ATPase decreased after intensive exercise. The study demonstrated that intensive exercise could induce heavy oxidative damages, and resveratrol enhance brain antioxygen and ATP enzymatic activity and lessen oxidative damages of brain.

关 键 词：白藜芦醇 大强度运动 抗氧化酶 一氧化氮 一氧化氮合酶 ATP酶 脑 

分 类 号：G804.7[文化科学—运动人体科学]