一氧化氮和过氧化氢对万寿菊不定根形成的影响  

Effect of Nitric Oxide and Hydrogen Peroxide on Adventitious Root Development of Marigold

作　　者：张美玲[1,2] 廖伟彪[3,4] 肖洪浪[4] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]甘肃农业大学理学院,甘肃兰州730070 [3]甘肃农业大学农学院甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [4]中国科学院寒区旱区环境与工程研究所,甘肃兰州730000

出　　处：《中国沙漠》2012年第1期105-111,共7页Journal of Desert Research

基　　金：国家自然科学基金项目(31160398);甘肃省自然科学基金项目(1010RJZA211);中国博士后科学基金项目(20100470887);教育部科学技术研究重点项目(211182)资助

摘　　要：以万寿菊(Tagetes erecta L.)为试材,研究了一氧化氮(NO)和过氧化氢(H2O2)对植物不定根形成中的影响及其相互关系。结果表明,外源NO供体肖普纳(SNP)和H2O2可显著地促进万寿菊外植体不定根的形成,且呈现明显的剂量效应,最适的SNP和H2O2浓度分别为50μM和200μM。NO和H2O2共同处理的万寿菊不定根的数量和根长显著高于NO或H2O2单独处理。NO在H2O2诱导万寿菊不定根形成的信号途径中起了重要作用;同时,H2O2包含在NO诱导不定根形成途径中。NO引起万寿菊外植体内源H2O2含量增加,H2O2亦促进内源NO积累。可见,NO和H2O2在万寿菊不定根形成过程中具有协同诱导效应,两者在促进不定根形成过程中可能通过互作反应提高各自的信号水平。Effect of nitric oxide（NO） and hydrogen peroxide（H2O2） on adventitious root development of marigold（Tagetes erecta L.） and their interrelationship were studied.Results showed that exogenous NO-donor-sodium nitroprusside（SNP） and H2O2,had significant positive effect on adventitious root development,and the effect was dose-dependent with a maximal biological response at 200 μM for H2O2 and 50 μM for SNP.The root number and length of explants treated with both H2O2 and NO were significantly higher than those of explants treated with H2O2 or NO alone.NO is essential for H2O2-induced rooting,and H2O2 is also involved in NO-induced rooting.NO treatment enhanced H2O2 levels in cells of explants hypocotyls,simultaneously external application of H2O2 induced NO generation in cells too.Thus,H2O2 and NO may act synergistically to mediate adventitious root generation of the explants and they may enhance each other＇s levels by their mutual amplification in that process.

关 键 词：一氧化氮 过氧化氢 万寿菊 不定根 

分 类 号：Q945[生物学—植物学]