深圳口岸国际旅行人员中HIV-1感染者耐药基因型检测分析  

Analysis on genotypes of HIV-1 drug resistance mutations among international travelers at Shenzhen ports

作　　者：刘胜牙[1] 朱玉兰[1] 刘春芳[1] 李微[1] 古莉冰[1] 张登峰[1] 俞杨[1] 

机构地区：[1]深圳国际旅行卫生保健中心,广东深圳518033

出　　处：《中国国境卫生检疫杂志》2011年第6期427-429,447,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：国家自然科学基金项目(81102174);国家质检总局科研基金项目(2009IK216)

摘　　要：目的对深圳口岸1996—2008年已确诊的68例人类免疫缺陷病毒(HIV-1)感染者进行耐药性突变分析,以了解深圳口岸国际旅行者HIV-1耐药性毒株的流行情况。方法应用巢式RT-PCR扩增HIV-1聚合酶基因,对所获得的PCR产物直接测序,再将所获得的序列与国际耐药数据库比对,辨别耐药性突变位点。结果在68例HIV-1感染者中,经巢式RT-PCR扩增并获得聚合酶基因序列68份。耐药分析结果表明,本研究人群未检测到蛋白酶抑制剂(PI)原发耐药突变;11例感染者发生反转录酶抑制剂(RTI)耐药基因突变,基因突变率为16.17%;其中核苷类和非核苷类反转录酶抑制剂的耐药突变率分别为7.35%(5/68)和8.82%(6/68)。结论本研究分析了深圳口岸国际旅行者HIV-1感染者耐药突变的流行情况,这对于我国的艾滋病预防和控制工作具有重要指导意义。Objective To understand the epidemiology of HIV-1 drug resistance strains,and to analyze drug resistance associated mutations of HIV-1 among 68 plasma samples at Shenzhen ports from 1996 to 2008.Methods HIV-1 pol gene was amplified by nest RT-PCR,PCR products were directly sequenced.Drug resistance associated mutations in protease and reverse transcriptase regions were analyzed with Standford University HIV/AIDS Drug Resistance Database.Results The mutations conferring secondary resistance to PI were detected in 11 of 68 patients,（16.17%）,and none of the isolates had major resistance mutations in the protease gene.The resistance-associated to NRTI and NNRTI in the pol gene were 5（7.35%）and 6（8.82%）,respectively.Conclusion This study reveals the emergence of drug resistance associated mutations of HIV-1 currently circulating among international travelers.It is believed that this information may help to guide recommendation for strategies of AIDS prevention and control in China.

关 键 词：艾滋病毒HIV-1 感染者 基因 耐药突变 国际旅行者 口岸 

分 类 号：R512.91[医药卫生—内科学]