毛细管气相色谱法定量分析地米尿素冰片乳膏中的龙脑和异龙脑  

Quantitative Analysis of Borneol and Isoborneol in Dexamethasone Urea/Borneol Cream by Capillary GC

作　　者：张辉[1] 陈文秋 余敏灵 

机构地区：[1]四川省医学科学院·四川省人民医院皮肤病性病研究所,成都610031 [2]乐山市食品药品检验所,四川乐山614000

出　　处：《中国药房》2012年第5期448-450,共3页China Pharmacy

摘　　要：目的:建立地米尿素冰片乳膏中龙脑和异龙脑的定量分析方法。方法:采用毛细管气相色谱法。色谱柱为聚乙二醇20000石英毛细管柱;柱温为140℃;进样口温度为180℃;载气为氮气,流速为1.2mL.min-1;火焰离子化检测器温度为180℃;进样量为1μL。结果:龙脑和异龙脑进样量线性范围分别为113.03～1808.48、86.51～1384.16ng(r=0.9998、0.9991,n=6);平均回收率分别为97.9%和98.2%(RSD=0.8%、0.7%,n=9)。结论:所建立的分析方法快速、准确,适用于地米尿素冰片乳膏中龙脑和异龙脑的定量分析。OBJECTIVE： To establish a method for quantitative analysis of borneol and isoborneol in Dexamethasone urea/bor- neol cream. METHODS： Capillary GC was adopted. The determination was carried out on a PEG20000 capillary column. The col- umn temperature was 140 ℃ and using nitrogen as carrier gas at the flow rate of 1.2 mL. min-2. The temperature of hydrogen flame ionization detector was 180℃ and injection volume was 1 gL. RESULTS： The linear ranges were 113.03-1 808.48 ng for borneol （r=0.999 8, n=6） and 86.51~1 384.16 ng for isoborneol （r=0.999 1, n=6）. Average recoveries were 97.9% and 98.2% （RSD=0.8% and 0.7%,n=9）, respectively. CONCLUSION： The method is rapid, accurate and suitable for quantitative analysis of bomeol and isobomeol in Dexamethasone urea/borneol cream.

关 键 词：毛细管气相色谱法 地米尿素冰片乳膏 龙脑 异龙脑 定量分析 

分 类 号：R927.2[医药卫生—药学]