艾灸预刺激对AD大鼠细胞周期调节因子CyclinA,p21/cip的影响  

Influence of Pre-stimulation with Moxibustion on Cells Period Adjustment Factor-CyclinA P21/cip in AD Models Rats

作　　者：刘静[1] 杜艳军[1] 李婧[1] 刘若兰[1] 王晓兰[1] 孙国杰[1] 

机构地区：[1]湖北中医药大学针灸骨伤学院,湖北武汉430061

出　　处：《时珍国医国药》2011年第11期2612-2613,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30901925);武汉市晨光科技计划(No.200850731347);湖北省自然科学基金(No.2007ABA084)

摘　　要：目的通过观察艾灸预刺激对阿尔茨海默病(AD)模型大鼠细胞周期调节因子CyclinA、p21/cip的影响,以探讨艾灸防治AD的作用机理。方法将SD大鼠随机分为4组,即正常组、假手术组、模型组、预艾灸组,每组10只,以双侧海马注入Aβ1-42制作AD大鼠模型,造模前预艾灸组采用艾条温和灸"百会"、"肾俞"穴,采用免疫组化法测定大鼠海马区CyclinA、p21/cip的阳性细胞数。结果通过3个疗程的预艾灸治疗,预艾灸组大鼠海马区CylinA阳性细胞数较模型组有显著降低(P<0.01),p21/cip阳性细胞数有显著增强(P<0.01)。结论艾灸预刺激对异常表达的细胞周期相关蛋白有一定的调节作用,从而减少AD的病理改变或相关的细胞凋亡,达到防治AD的目的 。Objective By investigating the effect of pre - stimulation with moxibustion on Cells Period Adjustment Factor - CyctinA,p21/cip in rat models of Alzheimer＇s disease so as to research into its underlying mechanism in preventing and controlling disease in the rats. Methods All animals were randomly divided into four groups, normal group, sham - operation group, model group, pre - moxibustion group. AD rats were induced by injecting β - Amyloid 1 - 42 （ Aβ1 - 42 ） into the double hippocampus. Meanwhile,immunohistochemistry was used to determine the levels of expression of rat＇s CyclinA,p21/cip in rat＇s hippocampus area. Results Compared with model group, pre - moxibustion group rat lowered obviously in CyclinA expression of hippocampus area and increased evidently in p21/cip expression. Conclusion These results imply that pre -stimulation with moxibustion can reverses the expression of cell cycle - related protein in hippocampus area, reduce consequently the pathological changs of Alzheimer g Disease.

关 键 词：阿尔茨海默病 艾灸 预刺激 CYCLINA p21/cip 

分 类 号：R285.5[医药卫生—中药学]