玳玳果黄酮有效部位中柚皮苷含量的高效液相色谱测定  

Determination of Naringin in the Total Flavonoids of Citrus Aurantium L.var Daidai Tanaka Fruits by HPLC

作　　者：刘永静[1] 陈丹[1] 黄庆德[1] 邱红鑫[1] 吴晓青[1] 

机构地区：[1]福建中医药大学药学院,福建福州350108

出　　处：《时珍国医国药》2011年第11期2649-2650,共2页Lishizhen Medicine and Materia Medica Research

基　　金：福建省自然科学基金(No.2010J01189);福建省科技厅重点项目(No.2010Y0030);福建省科技计划项目(No.2010Y2004)

摘　　要：目的建立高效液相色谱法测定玳玳果黄酮有效部位中柚皮苷含量的方法。方法色谱柱为250-4 lichrocart C18柱,流动相为乙腈-0.1%磷酸水溶液(20∶80),流速为1.0 ml/min;检测波长为283 nm。结果柚皮苷浓度在0.008 91～0.089 10 mg/ml范围内呈良好的线性关系,平均回收率为99.7%,RSD为1.60%。结论所建立的方法精密度、稳定性及重复性良好,可用于玳玳果黄酮有效部位的质量控制。Objective To establish an HPLC method for determination of Naringin in the total flavonoids of Citrus Aurantium L.var daidai Tanaka fruits. Methods 250-4 lichrocart C_18 column（250 mm ×4.6 mm,5 μm） was used with the mobile phase consisted of acetonitrile-0.1% phosphoric acid solution（20∶80）at a flow rate of 1.0 ml /min,and the detection wavelength was set at 283 nm. Results The calibration curve of Naringin was in good linearity over the ranges of 0.00891～0.08910 mg/ml（r=0.999 5）,and the average recovery was 99.70% with RSD at 1.60%. Conclusion The specificity of HPLC method is high and the method can be used to control the quality of total flavonoids of Citrus Aurantium L.var Daidei Tanaka Fruit.

关 键 词：玳玳果黄酮有效部位 柚皮苷 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]