白草花叶病毒承德玉米分离物3'-cDNA片段序列分析  

Sequencing and analysis of the 3′-terminal genome of Pennisetum mosaic virus isolates from maize plants in Chengde

作　　者：崔小雯[1] 高波[1] 许斐斐[1] 李向东[1] 张春庆[2] 苗洪芹[3] 

机构地区：[1]山东农业大学植物保护学院植物病理学系,泰安271018 [2]山东农业大学农学院,泰安271018 [3]河北省农科院植保所,保定071000

出　　处：《植物病理学报》2012年第1期32-36,共5页Acta Phytopathologica Sinica

基　　金：转基因生物新品种培育重大专项(2009ZX08003-014B);山东省科技攻关项目(2009GG10009021);山东省现代农业产业技术体系

摘　　要：采自河北承德11个表现矮花叶症状的玉米样品,用甘蔗花叶病毒(Sugarcane mosaic virus,SCMV)和白草花叶病毒(Pennisetum mosaic virus,PenMV)简并引物扩增了基因组3'端约2.1 kb的片段并进行测序。Blast结果表明其中8个样品含有PenMV。扩增到的PenMV序列均为2 135 nt,包括部分NIb基因(985 nt)、完整的CP基因(909 nt)和3'-UTR(241nt)。这8个分离物CP基因和3'-UTR与GenBank上其他PenMV分离物相应序列的核苷酸一致率分别为89.8%～93.4%和95.9%～97.9%。根据扩增的2 135 nt序列和CP基因序列构建系统发育树,8个分离物与GenBank上其他PenMV分离物都分为2个组:山西组和承德组。重组分析表明CD9的CP基因存在重组。Totally 11 maize samples showing dwarf mosaic symptoms were collected from Chengde, Hebei Province. The 3＇-terminal 2.1 kb genomic fragments were amplified with degenerate primers for Sugarcane mosaic virus （SCMV） and Pennisetum mosaic virus （PenMV） and then sequenced. The Blast results showed that eight samples were infected with PenMV. The cloned genome of these eight isolates were all 2 135 nucleotides （nt） long, including partial NIb gene （985 nt）, complete CP gene （909 nt） and the 3＇-UTR （241 nt）. The CP gene and 3＇- UTR shared nt identities of 89.8% -93.4% and 95.9% -97.9%, respectively, with the corresponding PenMV sequences available in the GenBank. In the phylogenetic trees constructed with the 3＇-terminal 2 135 nt sequence and CP gene, all the PenMV isolates were divided into two groups： Shanxi group （SX） and Chengde group （CD）. Recombination event was detected in the CP gene of isolate CD9.

关 键 词：白草花叶病毒 玉米 系统发育分析 重组 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治] Q939.46[农业科学—植物保护]