拮抗链霉菌B28的分类鉴定及其生防作用研究  

Biocontrol effect and taxonomy of antagonistic Streptomyces strain B28

作　　者：申屠旭萍[1] 于冰[1] 边亚琳[1] 胡靓[1] 马正[1] 童朝明[2] 俞晓平[1] 

机构地区：[1]浙江省生物计量及检验检疫技术重点实验室,中国计量学院生命科学学院,杭州310018 [2]杭州市农业科学研究院,杭州310024

出　　处：《植物病理学报》2012年第1期105-109,共5页Acta Phytopathologica Sinica

基　　金：浙江省科技计划项目(2010C32063);浙江省重点创新团队自主设计项目(2011R09027-03);杭州市科技计划项目(20101032B27、20110232B04);大学生科技创新活动项目(2010R409025)

摘　　要：目前植物真菌病害的防治仍以化学药剂为主。化学农药虽然具有毒力强、见效快等特点，但长期大量使用化学农药带来了环境污染、农药残留和病原菌的抗药性等诸多问题。因此，研制高效、低毒、和对环境安全的生物农药已迫在眉睫。放线菌是一类有着广泛实际用途的微生物资源，An antagonistic Streptomyces strain B28, isolated from the soil collected in Tianmu mountain of Zhejiang Province, was identified as Streptomyces diastatochromogenes by morphological , physiological ＆ biochemical characteristics and 16S rDNA sequence alignment. Antagonistic study indicated that its secondary metabolite toyocamycin had an antifungal activity on the mycelial growth of seven species of plant pathogenic fungi. The results showed that toyocamycin possessed marked inhibitory activity against Rhizoctonia solani and the ECso value was 0.58 μg/mL. The effective of toyocamycin against Botrytis cinerea and Fusarium oxysporum f. sp. cucumerinum was much higher than that against Colletotrichum gloeosporioides, the EC50 value of toyocamycin in inhibiting B. cinerea, F. oxysporum and C. gloeosporioides were 13.65μg/mL, 12.33 μg/ mL, and 30.15 μg/mL respectively.

关 键 词：拮抗链霉菌 分类鉴定 生防 植物真菌病害 化学农药 微生物资源 化学药剂 环境污染 

分 类 号：S482.28[农业科学—农药学]