从腺梗豨莶中分离得到的一种具有细胞毒活性的新氨基甲酸酯(英文)  

A new carbamate with cytotoxic activity from the aerial parts of Siegesbeckia pubecens

作　　者：刘佳[1,2] 陈荣[2] 聂煜[3] 冯林[2] 李海岛[2] 梁敬钰[1] LIU Jia;CHEN Rong;NIE Yu;FENG Lin;LI Hai-Dao;LIANG Jing-Yu(Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China;Department of Liye Science and Technology,China Pharmaceutical University,Nanjing 210009,China;Jiangsu Simcere Pharmaceutical R&D Co.,Ltd.,Nanjing 210042,China)

机构地区：[1]中国药科大学天然药物化学教研室,南京210009 [2]江苏先声药物研究有限公司,南京210009 [3]中国药科大学生命科学与技术教研室,南京210042

出　　处：《中国天然药物》2012年第1期13-15,共3页

基　　金：supported by the Natural Science Foundation of Jiangsu Province of China(No.BK2009470)~~

摘　　要：目的:对腺梗豨莶(Siegesbeckia pubescens)地上部分进行化学成分的研究。方法:运用萃取、结晶及各种柱层析方法进行分离纯化, 并通过理化鉴别和波谱分析鉴定化合物结构。此外, 测定了化合物 1 对 A549 人肺癌细胞的细胞毒活性。结果:从腺梗豨莶中分离鉴定了4个化合物,分别为(E)-3-(3-oxobut-1-enyl)phenyl dimethylcarbamate(1),ent-2-oxo-15,16,19-trihydroxypimar-8(14)-ene(2),16-acetylkirenol(3)和3,7-dimethylquercetin(4)。结论:化合物1为新化合物,MTT细胞毒活性显示其 IC50为58 μg mL-1。AIM： To study the chemical constituents of Siegesbeckia pubescens. METHODS： The chemical constituents were isolated by extraction, crystallization and various chromatographic methods, and the chemical structures were elucidated on the basis of spectral analysis. In addition, the cytotoxic activity of compound 1 was evaluated using human lung cancer cell A 549. RESULTS： Four compounds were obtained, and their structures were identified as （E）-3-（3-oxobut-l-enyl）phenyl dimethylcarbamate （1）, ent-2-oxo-15, 16, 19-trihydroxypimar-8（14）-ene （2）, 16-acetylkirenol （3）, 3, 7-dimethylquercetin （4）. CONCLUSION： Compound 1 is a new carbamate, and the IC50 in MTT method of compound 1 was 58 μg·mL-l.

关 键 词：腺梗豨莶 化学成分 细胞毒 

分 类 号：R284.1[医药卫生—中药学]