血液透析患者低密度脂蛋白受体相关蛋白RsaI位点基因多态性对血脂水平的影响  

Detection of Rsa I gene polimorphism of low density lipoprotein receptor related protein and its influence on serum lipid level in hemodialysis patients

作　　者：王玉新[1] 张以勤[1] 叶婧[1] 陆元善[1] 徐琴君[1] 邹和群[1] 

机构地区：[1]厦门市第二医院肾内科,福建361021

出　　处：《国际泌尿系统杂志》2012年第1期8-12,共5页International Journal of Urology and Nephrology

摘　　要：目的探讨血液透析患者脂代谢紊乱的临床特征及低密度脂蛋白受体相关蛋白（LRP）外显子3RsaI位点基因多态性对脂代谢的影响。方法生化方法检测血液透析患者血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDLC）、低密度脂蛋白胆固醇（LDLC）、载脂蛋白（Apo）A1、ApoB、ApoE及脂蛋白（Lp）（a）水平，多聚合酶链反应－限制性片断长度多态性方法（PCR—RFLP）检测LRP外显子3RsaI位点基因多态性。健康体检者372例作为对照组。结果血液透析患者脂代谢紊乱主要表现为血清TG水平显著增高，HDLC水平显著降低。血清TG水平高于正常者为33％，HDLC低于正常者为10．4％。偏相关回归分析显示，TG水平与血清ALB水平、透析时体外循环血流量显著相关；HDLC与KT／V显著相关。血液透析患者高血压的发生率为73．6％，心血管疾病为25％。伴心血管疾病组TG水平显著高于无心血管疾病组，伴高血压组与无高血压组血脂水平无显著差异。LRP基因多态性分析显示，LRP外显子3基因型C／C与等位基因C发生频率较高，病例组与对照组间无显著差异。对照组基因型C／T者血清TC、TG、LDLC水平高于基因型为C／C者，后者具有显著性差异；病例组无显著差异病例组外显子3RsaI位点不同基因型间血脂水平无显著差异。结论血液透析患者脂代谢紊乱主要表现为血清TG、ApoB水平显著增高，HDLC等指标显著降低。伴心血管并发症的患者TG水平显著高于无并发症的患者。TG水平与血清ALB水平、透析时体外循环血流量显著相关；HDLC与KT／V显著相关。LRP外显子3RsaI位点基因多态性对正常人群与血液透析患者血脂水平有不同的影响。Objectives To investigate the characteristics of dislipidemia and influence by low density lipo-protein receptor related protein （LRP） exon 3 Rsa I gene polymorphism in hemodialysis patients. Methods Se -rum total cholesterol （TC）, triglyeeride （TG）, low density lipoprotein cholesterol （LDLC）, high density lipoprotein cholesterol ＇（HDLC）, apolipoproteins （Apo A1, B, E）and lipoprotein（a） [ Lp（a） ] were measured, polymerase chain reaction - restriction fragment length polymophism （ PCR - RFLP） was used to detect LRP gene polymorphism in hemodialysis patients. Results The serum levels of TG and ApoB were higher significantly in the hemedialysis patients compared to contrals, and the levels of TC, LDLC, HDLC were lower compared to controls. Patients with cardiovascular disorder had higher TG level compared to patients without the disorder. The data of LRP polymorphism detected by PCR - RFLP showed that the allele frequency and the distribution of the LRP genotype of tetranueleotide reapeat sequence and exon 3 did not differ significantly between controls and hemodialysis patients. There was no statistic difference in serum lipid level between hemodialysis patients with different genotype of LRP exon 3. Conclusions Hemodialysis patients were liable to develop hypertrglyeridemia and hypoeholesteroldemia. TG level might related to the development of cardiovascular disorder in hemedialysis patients. There is different influence of LRP Rsa I gene polymorphism on lipid levels between controls and hemodialysis patients.

关 键 词：血液透析 脂蛋类 LDL 脂类 多态现象(遗传学) 

分 类 号：R459.5[医药卫生—治疗学]