蛋白激发子基因peaT1转基因棉花的获得及初步鉴定  

Identification of Transgenic Cotton with a Protein Elicitor-encoding Gene peaT1

作　　者：唐宏琨[1] 曾洪梅[1] 杨秀芬[1] 李国媛[1] 盛德鹏[1] 袁京京[1] 邱德文[1] 

机构地区：[1]中国农业科学院植物保护研究所生物防治农业部重点开放实验室,北京100081

出　　处：《生物技术通报》2012年第1期60-64,共5页Biotechnology Bulletin

基　　金：转基因重大专项(2008ZX08010-004;2009EX08001-018B)

摘　　要：将来源于极细链格孢菌的蛋白激发子基因peaT1克隆到植物表达载体pCAMBIA2300上,成功构建了含有增强子和多联终止子的植物表达载体pCAMBIA2300-G4AS-peaT1。通过花粉管通道法转化棉花,经卡那霉素抗性筛选、PCR和RT-PCR检测获得一株转基因棉花植株,其植株茎粗和成铃数均高于非转基因对照株。The protein elicitor-encoding gene peaT1 obtained from Alternaria tenuissima was inserted to pCAMBIA2300 to construct the plant expressive vector pCAMBIA2300-G4AS-peaT1 which contains enhancer and poly-terminator.Then,the resulting vector was transferred into cotton by pollen-tube pathway method.The positive transgenic line was screened with kanamycin resistance,PCR,and RT-PCR.The resultant plant got more cotton bolls and the stem diameter was higher compared with the control.

关 键 词：蛋白激发子 peaT1 花粉管通道 棉花 

分 类 号：S562[农业科学—作物学]