C2C12成肌细胞诱导肌性分化过程中miR-101a的表达  

The Expression of miR-101a in C2C12 Myoblast During Myogenic Differentiation

作　　者：李粉[1] 王丽[1] 郑艳艳[1] 陈华群[1] 

机构地区：[1]南京师范大学生命科学学院江苏省分子医学生物技术重点实验室,南京210046

出　　处：《生物技术通报》2012年第1期116-119,共4页Biotechnology Bulletin

基　　金："973"计划项目(2009CB941600)

摘　　要：以C2C12成肌细胞为模型,在分化培养基中诱导C2C12建立体外肌性细胞分化模型。以poly(A)3'-端加尾和实时定量PCR方法研究miR-101a在C2C12细胞分化过程中的表达情况。结果发现,在细胞转入分化培养基进行肌性分化的1-5 d中,miR-101a的表达量逐渐增加,提示miR-101a可能在肌肉发生中发挥调控作用。In order to establish muscle differentiation model,the C2C12 myoblast cells were incubated in differentiation medium（DM） and induced to myogenic differentiation.The expression of mature miR-101a was examined by poly（A） tail addition at 3＇-end and Real-time PCR method.The results showed that the level of mature miR-101a was gradually increased during the induction time from 1 day to 5 days after the cells changed to DM,suggesting that miR-101a may play regulatory roles in myogenesis.

关 键 词：C2C12C成肌细胞 实时定量PCR miR-101a 肌分化 

分 类 号：Q445[生物学—生理学]