人胎盘糖皮质激素受体与宫缩的关系  

Relationship between human placental glucocorticoid receptor and uterine contraction

作　　者：于坤[1] 杨莉[1] 陈明[1] 

机构地区：[1]遵义医学院附属医院产科,贵州遵义563000

出　　处：《中国妇幼保健》2012年第3期428-432,共5页Maternal and Child Health Care of China

摘　　要：目的:检测人胎盘组织中糖皮质激素受体水平,探讨其与妊娠子宫收缩的关系。方法:收集2010年1～8月在遵义医学院附属医院产科住院的60例单胎、足月妊娠、初产妇为研究对象,根据宫缩情况分为3组,每组各20例。取产后胎盘母体面(脐带附着处)约2 cm×2 cm×1 cm组织,标本经处理后采用免疫组化Envision法检测胎盘中糖皮质激素受体表达水平,并进行图像分析。结果:①免疫组化结果显示:宫缩正常组人胎盘滋养叶细胞胞浆中糖皮质激素受体强阳性表达,为黄色或棕黄色染色,原发性宫缩乏力组与无宫缩组人胎盘滋养叶细胞胞浆透明,糖皮质激素受体几乎无表达。②宫缩正常组人糖皮质激素受体阳性颗粒平均光密度值为0.335±0.119,宫缩乏力组为0.185±0.074,无宫缩组为0.182±0.077,3组值两两比较,宫缩正常组比原发性宫缩乏力组及无宫缩组高,P<0.05,差异有统计学意义,原发性宫缩乏力组与无宫缩组比较,P>0.05,差异无统计学意义。③宫缩正常组20例,糖皮质激素受体阳性表达16例,阳性表达率为80.0%;原发性宫缩乏力组中阳性表达2例,阳性表达率为10.0%;无宫缩组阳性表达1例,阳性表达率为5.0%,宫缩正常组与原发性宫缩乏力组及无宫缩组比较,P<0.05,差异均有统计学意义,卡方值分别为χ2=19.798,χ2=23.018,原发性宫缩乏力组与无宫缩组比较,P>0.05,差异无统计学意义。结论:糖皮质激素受体在人胎盘中的表达水平与宫缩强度有密切关系,可能与早产及产力异常的发生有关。Objective：To detect the level of glucocorticoid receptor in human placental tissue,explore its relationship with uterine contraction. Methods：Sixty full-term primiparous women of single pregnancy who hospitalized in obstetrical department of the hospital from January to August in 2010 were collected as study objects,then they were divided into three groups according to uterine contraction conditions,20 women in each group.A piece of postpartum placental specimen（volume： 2 cm×2 cm×1 cm） was obtained from the maternal surface of placenta（near the adherent place of umbilical cord） in each group,after treatment,immunohistochemical Envision method was used to detect the expression level of glucocorticoid receptor in placenta,image analysis was carried out. Results：Immunohistochemical results showed strongly positive expression of glucocorticoid receptor in cytoplasm of human placental cytotrophoblasts in normal uterine contraction group,which manifested as yellow or brown yellow particles;in primary uterine inertia group and non-uterine contraction group,the cytoplasm of human placental cytotrophoblasts was pellucid,glucocorticoid receptor barely expressed.The mean optical density values of positive particles of glucocorticoid receptor in normal uterine contraction group,primary uterine inertia group,and non-uterine contraction group were（0.335±0.119）,（0.185±0.074）,and（0.182±0.077）,respectively;the mean optical density value in normal uterine contraction group was significantly higher than those in primary uterine inertia group and non-uterine contraction group（P0.05）,but there was no significant difference in the mean optical density value between primary uterine inertia group and non-uterine contraction group（P0.05）.The positive expression rates of glucocorticoid receptor in normal uterine contraction group,primary uterine inertia group,and non-uterine contraction group were 80.0%（16/20）,10.0%（2/20）,and 5.0%（1/20）,respectively;there was significant difference betw

关 键 词：子宫收缩 糖皮质激素受体 糖皮质激素 妊娠 胎盘 

分 类 号：R714.4[医药卫生—妇产科学]