AB-8大孔吸附树脂纯化双黄连中金银花、连翘提取物工艺  

Purification Technology of Extracts from Lonicerae japonicae and Forsythiae suspensa in Shuanghuanglian by AB-8 Macroporous Resin

作　　者：李淑芳[1] 王淑美[2] 梁生旺[2] 王战红[3] 

机构地区：[1]郑州市第三人民医院,郑州450000 [2]广东药学院,广州510000 [3]河南中医学院,郑州450000

出　　处：《中国实验方剂学杂志》2012年第2期4-7,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073024);广东省自然基金项目(945102240);河南省自然基金项目(0411044800)

摘　　要：目的:优化AB-8大孔吸附树脂纯化双黄连中金银花、连翘提取物的工艺。方法:以提取物中总酚、总黄酮的含量为考察指标,从树脂径高比、吸附浓度、吸附流速、洗脱剂浓度等方面对大孔吸附树脂纯化工艺进行优化。结果:最佳纯化工艺为金银花、连翘提取物,加蒸馏水稀释成1.5 g.mL-1(生药量),树脂柱径高比1∶10,最大吸附量与树脂体积比为1∶3.3,水洗3倍树脂体积,4BV 70%乙醇洗脱。结论:AB-8大孔吸附树脂纯化金银花、连翘提取物的最佳工艺稳定可行。Objective：To optimize purification technology of extracts from L.japonicae and F.suspensa in Shuanghuanglian by AB-8 macroporous resin.Method： The content of total phenolic and total flavonoids were used as indexes.Purification technology was optimized from proportion of diameter-height,adsorption concentration,adsorption speed and eluent concentration,etc.Result： Optimum purification technology was as follows： extracts of L.japonicae and F.suspensa were diluted to concentration was 1.5 g·mL-1by water,ratio of diameter-height 1∶10,proportion of maximum adsorption to resin volume was 1∶3.3,eluted by 3 BV of water,4 BV 70% ethanol as the eluent.Conclusion： This process of purification technology was stable and feasible.

关 键 词：双黄连 大孔吸附树脂 纯化工艺 

分 类 号：R283.6[医药卫生—中药学]