怀山药及非药用部位总黄酮含量测定  

Content Determination of Total Flavonoids in Dioscorea oppositea and Non-medicinal Parts

作　　者：吕鹏[1] 贾秀梅[1] 张振凌[1] 陈益清[1] 

机构地区：[1]河南中医学院药学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第2期65-68,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十二五"科技重大专项课题(2011BAI06B04)

摘　　要：目的:为充分利用怀山药资源,避免资源的浪费,研究怀山药各部位总黄酮的供试品制备方法,探讨怀山药各部位的总黄酮含量的变化。方法:采用乙醇超声提取法,正交设计实验优选怀山药中总黄酮的供试品制备方法,分光光度法测定含量。结果:怀山药总黄酮的供试品制备方法为95%乙醇15 mL超声提取3次,每次提取45 min。分光光度法其测定怀山药叶中黄酮含量最高,其次是生山药皮粉、藤茎、山药零余子。结论:怀山药各部位总黄酮含量有显著差异。Objective：Studying the best extraction method of flavonoids and its change in various parts of Dioscorea opposita Thunb to take full advantage of yam resources and to avoid wastage.Method： Using ethanol ultrasonic extraction method and orthogonal experimental design to select the best extraction method of total flavonoids and determing the content by spectrophotometry.Result： The extraction method of total flavonoids in D.opposita is： ultrasonic extraction with 15 mL of 95% ethyl alcohol 3 times,each time 45 min.The leaf flavonoid content is the highest,followed by skin raw yam flour,cane,yams Bubil.Conclusion： Total flavonoid content in D.opposita in different parts were significantly different.

关 键 词：怀山药 总黄酮 含量测定 分光光度法 

分 类 号：R284.1[医药卫生—中药学]