HPLC测定齐酞酸钠含量及其有关物质  

HPLC Determination of the Content of Oleanolic Acid 3β-phthalic Monoester Disodium Salt and its Related Substances

作　　者：麻秀萍[1] 蒋朝晖[1] 贾宪生[1] 陈聪[1] 邓华湘[1] 

机构地区：[1]贵阳中医学院药学系,贵阳550002

出　　处：《中国实验方剂学杂志》2012年第2期89-91,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科技厅攻关项目(黔科合字[1998]9号)

摘　　要：目的:用高效液相色谱法测定齐酞酸钠的含量及其有关物质。方法:采用Eclipse XDB-C18色谱柱(4.6 mm×150mm,5μm),以甲醇-乙腈-0.1%磷酸水溶液(80∶10∶10)为流动相,流速1.0 mL.min-1,柱温35℃,检测波长210 nm。结果:齐酞酸钠与其相邻杂质峰能完全分离,齐酞酸钠在0.052 5～0.997 5μg进样量与峰面积呈良好线性关系(r=0.999 9),最低检出限为3 ng;精密度良好(RSD 0.38%),平均回收率为99.53%(n=9)。结论:该方法简便、准确、专属性好、灵敏度高,可用于齐酞酸钠的含量及有关物质测定。Objective：To determine the content and the related substances of oleanolic acid 3β-phthalic monoester disodium salt（OAPES） by HPLC.Method： The separation was performed on Eclipse XDB-C18 column（4.6 mm×150 mm,5 μm）,column temperature at 35 ℃ with mobile phase of methanol-acetonitrile-0.1% phosphoric acid solution（80∶10∶10）.The flow rate was set at 1.0 mL·min-1 with detection at 210 nm.Result： There was a good linear relationship within the range of 0.052 5-0.997 5 μg（r=0.999 9）,the least detective range is 3 ng.The precision in a day is good（RSD 0.38%）.The average recovery was 99.53%（n=9）.Conclusion： The method is simple,accurate,specific and sensitiveness for the determination OAPES content and its related substances.

关 键 词：齐酞酸钠 含量 有关物质 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]